Child Marriages A human rights issue - 2995 Words

Most of the world knows the Muslim Middle East as a place for coveting oil, having a captivating culture and spicy cuisine. Yet the Middle East is also a place with a history filled with continuous civil wars, poverty, and social and political turbulence. In these strange lands it is often unclear who really makes the laws, a place where more children can be found begging on the street then in school. The fate of young village girls rest in the hands of tribal chiefs, within the home older brothers and fathers are the law. In the Middle Eastern Countryside women are not taught to make choices. At their age they do not ask questions. All children are delivered at home, their mothers’ laying on a woven mat swearing suffering, terribly†¦show more content†¦Ã¢â‚¬Å"According to Yemeni law, it is difficult for you to file a complaint against your husband and your father†(Ali, 81). Since children are born without identification documents, marriage is a contract signed and unanimously approved by the men in the family. The marriage of young girls is traced to an Ancient Tradition. Upon asking her mother, â€Å"Whatever did I do to deserve this?†(Ali, 91), And why she was being married off and later beaten, and raped by her new husband her mother responded, â€Å"That’s how life is, Nujood: all woman must endure this; we have all gone through the same thing†(Ali, 96). The problem with the concept of child marriages is that it steams from a seemly pure and honest source . In these villages â€Å"There is even a tribal proverb that says, ‘to guarantee a happy marriage, marry a nine-year-old girl†(Ali, 75). With ancient proverbs saying to marry young woman, there really is no room for debate. Upon Nujood’s mother asking her father his response was, â€Å"Too young? When the prophet Mohammed wed Aisha, she was only nine years old† (Ali, 54). Within the Qur’an the life of the prophet Mohammed is written with detail explicitly describing his marriage and marriage consummation to his own young bride. The codependent relationship between religion, marriage, and politics is extremely intricate. In Islamic culture as explored in Sex, Marriage, and Family in World Religions â€Å"TheShow MoreRelatedStruggle for Children’s Rights987 Words   |  4 Pagesof a 10-year-old wife - who was suffering from child marriage (Worth, â€Å"Voices Child Marriage Yemen†). Child marriage is a terrible scenario for children, especially for little girls. They are forced to marry, before they are mature enough due to social norms, traditions and their family members, whom they are depending on. According to statistics in 2013, in Bangladesh, the child marriage prevalence under the age of 18 is 66%. 240 among 100,000 child wives died, when they gave birth. In Niger, thisRead MoreThe Issue Of Child Marriage1637 Words   |  7 PagesUnited States, marriage is seen as a sacred and consensual institution, but in developing countries, many children are introduced to marriage in a different manner. According to the United Nations Population Fund, one girl in every three is married before the age of eighteen in developing countries (Child Marriage, 1). Are the measures previously taken by the International Law community that attempt to put and end to child marriages an effective measure to stop this violation of human rights? I argue thatRead MoreThe Sexual Offences Bill 2015 Essay1255 Words   |  6 Pagesvery strong link to the subject of child marriage in Nigeria hence the reason of its examination as it relates to child marriage and the CRA in line with the legal implications if it were to be made law. The SOB has left a trail of controversy with regards to its provisions on matters such as child marriage, age of sexual consent and pedophilia. To start with, section 7(1- 4) of the Bill states; (1) A person who commits an act which causes penetration with a child is guilty of an offence called defilementRead MoreThe Issue Of Child Marriage Essay1266 Words   |  6 PagesChild marriage is one of the hardest things a teenage child could face. It is basically a violation of children’s human rights. It is strictly prohibited by international law. Early marriage takes away the girls right to make important decisions about their sexual health and well being. Historically, patriarchal societies have marginalized women in various aspects across the world. Women disenfranchisement, in this way, is not a new phenomenon. However, with the passage of time women have been ableRead MoreThe Problem Of Human Rights1581 Words   |  7 PagesHuman rights have yet to become the major local ap proach to social justice as applied to the practice of early marriage in the Amhara Regional State, in Ethiopia. Currently, the language of rights in Ethiopia is intertwined with the formal law and the packaging of rights via government channels. There is negligible political space for the international human rights norms to penetrate the community without the intermediary role of government institutions and networks. This to the large part is attributedRead MoreGender And Gender Equality866 Words   |  4 Pageslens of women’s rights, gender equality is ever-present in modern day society. Formally, women’s rights can be defined as the rights that promote a position of legal and social equality of women in relation to men. This inconsistency remains a monumental barrier to human development. Females are discriminated against in many facets of life. For instance, it can be found within the fields of health, politics, business, and education. Gender Equality with Child Marriage Child marriages, defined asRead MoreThe Gay Marriage Should Be Legal947 Words   |  4 PagesDefending Gay Marriage During the last few years, homosexuality has become an important issue for debate. Moreover, homosexuals have taken their case further by claiming their right to marry. Same-sex marriage, usually known as â€Å"gay marriage†, is the marriage between two people from the same biological sex (Doskow1). Since 2000, eleven countries have approved the legalization of gay marriage worldwide: Netherlands, Belgium, Spain, Canada, South Africa, Norway, Sweden, Portugal, Iceland, ArgentinaRead MoreThe Role Of Globalisation On Emergence, Development And Outcomes Of Social Movements1515 Words   |  7 PagesIntroduction Human rights abuses are one of the major issues in society. With the rise of globalisation and emergence of international and domestic social movements the problem has been reduced however it is still alive. This essay aims to investigate the influence of globalisation on emergence, development and outcomes of social movements that seek to protect human rights. (60 words) Case One of the most serious human rights violations are forced child marriages. Child marriage is defined as aRead MoreChild Marriage And Low Levels Of Education989 Words   |  4 PagesAccording to a study determined by UNICEF, Niger has the highest rate of child marriage in the world. Countless Nigerien girls are taken out of school and married off every year, and can legally get married off at the age of just 15 with 75% of girls getting married before their eighteenth birthday (UNICEF). Activists sat that girls as young as seven are sold as brides in Niger. Across the globe, we see these rates are highest in this particular region, and it makes us wonder why it’s happening mostlyRead MoreThe Debate Over The Legalization Of Same Sex Marriage1264 Words   |  6 Pagesprevalent examples of this is the ongoing debate over the legalization of same-sex marriage within America. In the article â€Å"Supreme Court should make gay marriage a national right† by the editorial sta ff at the Boston Globe, the importance of the upcoming Supreme Court ruling on same sex marriage was stressed because it is a topic that has been dodged thus far. While numerous federal circuit courts have ruled on this issue, some in favor and some opposed, the legal dispute of whether the decision should

Benefits And Benefits Of Compensation Packages - 1182 Words

What is a compensation packages? Compensation packages are a group of different benefits that employees gain for employers. Compensation is needed for every business no matter what it is to be successful; I do not know anyone who would work for free. (â€Å"Cost-benefit analysis assesses the profitability of a financial endeavor by considering the present value of each cost and benefit. Present values are used under the assumption that money available now is worth more than money in the future. That is generally true in an economy with positive inflation, so values need to be discounted for this effect. Cost-benefit analysis use ratios for assessing the investment, but a positive cost-benefit ratio does not necessarily mean you have a positive expected return.†) (How to Determine Whether the Cost-Benefit Ratio Is Positive or Negative | Chron.com, 2015) Cost benefits are use to compare programs with many different units of programs. Cost- benefit ratio gives an indication stat ing if the benefits given outweighs the company. As far as cost benefit ratio this is what I found researching, (â€Å"A total of 148 patients acquired NI (88 before and 60 after the intervention). The Mantel-Haenszel stratified relative risk for NI in the period before the infection control program, compared with the postintervention period, was .61 (95% confidence interval: .53–.69). By applying the preintervention stratum-specific rates of infection to the days-at-risk in the postintervention period, anShow MoreRelatedEmployee Compensation And Benefits Packages Essay1245 Words   |  5 PagesEmployee Compensation and Benefits Organizations create compensation and benefits packages in order to attract the best talent. In today’s global economy it is imperative that organizations offer compensation packages that are competitive in order to recruit the very best talent in the world. However, in order to be successful, compensation packages must align with business strategies. Authors â€Å"Mathis, Jackson, and Valentine (2014) explain that an effective total rewards approach balances theRead MoreEmployee Compensation And Benefits Package Essay905 Words   |  4 PagesIntroduction – Employee Compensation and Benefits This paper will outline an employee compensation and benefits package for a new hire for a secretary for the department. First, it will describe the organization I chose for designating a compensation package. Next, this paper will develop an employee compensation and benefits package for this new position. This paper will outline an employee compensation and benefits package for a new hire for a secretary for the department. First, it will describeRead MoreBenefits And Compensation Package Of Medtronic910 Words   |  4 PagesIntroduction The assessment of the case study will analyze the benefits and compensation package of Medtronic. The validation of concerns will consider the importance of different employee demographics within the organization. Determination of issues that are most prominent based on the qualitative data. Key issues will also be identified based on employee satisfaction of the compensation package. The analysis will also include an external benchmarking of data from other companies within the industryRead MoreEmployee Compensation And Benefits Package944 Words   |  4 PagesEmployee Compensation and Benefits Package The organization for which I am designing the compensation package is a company that offers internet solution to customers in the domestic US market and the global market. The position that I am hiring is that of a company secretary. The secretary will be required to work in the office of the human resource manager and will handle all the papered and paperless documentation. The position comes with numerous benefits and packages as outlined herein. AfterRead MoreEmployee Compensation And Benefits Packages Essay1537 Words   |  7 PagesIntroduction In today’s competitive workforce, compensation and benefit packages plays a crucial role on recruitment and retention for both the organization and the employee. Bumpbie finds itself in a situation where it could positively affect its employee’s morale, turnover rate and longevity; by making a strategic decision to implement compensation and benefit packages that will encourage current workers to stay and entice new applicants. Money is not always the inherent reason businesses experienceRead MoreEmployee Compensation and Benefits Packages548 Words   |  2 PagesEmployee Compensation and Benefits Packages Introduction: A companys compensation and benefits package will likely be a great determinant of the quality of its personnel and the stake that these employees feel that they have in the hiring organization. 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Employee Training and Development In regards to training a strong, educated, and high-performance workforce, an up-to-date training plan that willRead MoreBenefits Of Compensation Packages At Analysts Compensation1935 Words   |  8 Pages The last but certainly not least significant way for a conflict of interest to arise with an analyst and his or her valuation is through that analyst’s compensation. The brokerage firms employing the analysts can take advantage of compensation packages to make analysts feel as if they are being shoved into a corner with little option but to only come up with buy-recommendations and optimistic research reports. Bonuses can be tied to the revenues brought in the the analyst’s employer, so decreasingRead MoreEffectively Managing Employee Compensation and Benefit Packages1289 Words   |  6 Pagesfor global organizations and the human resource department, is effectively managing employee compensation and benefits packages. 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Those that will be discussed here are term life insurance, whole life and universal life insurance, accidental death and dismemberment insurance

Business Strategy As The Direction For Organisations Business Essay Free Essays

string(140) " concern houses have 5 of import cardinal factors such as vision ends, strategic thought, basic accomplishments and linking with employees\." Scheme is the way and range of an administration over a long term. Strategy is a better manner of using the resources of the concern, run intoing the demands of the market, carry throughing the demands of the interest holders taking the concern in an effectual and profitable manner. Strategy is a long term program of action which is designed to accomplish a peculiar end or set of ends. We will write a custom essay sample on Business Strategy As The Direction For Organisations Business Essay or any similar topic only for you Order Now It will be best described as a direction program in order to accomplish the coveted ends. Business scheme is a long term attack to implement the programs for concern in order to accomplish the concern aim. It is concerned more with how a concern competes successfully in a peculiar market. How scheme can be constructed and carried out efficaciously, even by little concern: – Nowadays harmonizing to many companies many little concern houses face the world of planetary competition. Harmonizing to many companies reconstituting themselves helps them to confront competition in the universe markets. After carry oning organisational reappraisals over 100 concern associations and with the experience 18 old ages in the undertaking direction, i realise all the organisations attempts to implement their operations efficaciously. Harmonizing to a survey in 2004 ( little concern disposal ) 580900 little concern little houses opened in 2005 576200 stopping point, SBA states that 67 % of the new companies were able to last for at least 2 old ages, staying 44 % of the companies survive for at least 4 old ages. Four of import factors for competitory advantages: – 1. Vision 2. Basic accomplishments 3. Strategy believing 4. Conjuction with staff Vision: – It is an of import factor for a company to go on it on the path. Harmonizing to the analysis made by James Kouzes and Barry Posner, writers of the leading challange, on more than 60000 caputs of the organisation, they stated that the leaders who are effectual should animate a shared vision. A leader has to back up a clear vision in his leading of the organisation. Now yearss many of the directors are busy with the present jobs and missing clip for edifice of vision. This makes both employees and organisation to be left unfastened, with a shared vision little concerns gain a competitory advantage. Harmonizing to critics vision is the critical factor to keep success for little concerns. Vision is referred as sing the national resources in to the physical bounds. A leader who is effectual for a little concern should animate his squad with a vision which is familiar to carry through their demands. Core competences: – Basic accomplishments of the director should be clarified with workers altering yesteryear. In the average clip of restructuring, and organisation as to keep it core mission. Large companies calculating the market which will be quickly spread outing, which makes their employees confuse because the contradictions and uncertainnesss. Companies which attempts to make things that are beyond their organisational capacity, which makes them to confront the failure. Many companies which are seeking to vie with planetary competition are losing their basic accomplishments in the market. Harmonizing to Ck prahlad, writer of viing for the hereafter states that directors do non finish their undertakings to vie with rapid market alterations. He states that reconstituting initiative his defines as a dead terminal, reengineering removes unneeded work and better the advantage of procedures as it is defines as the social ends like client satisfaction or entire quality directed enterprise. Strategic Thinking: – Employees wanted their leaders to be important in the market and competition which is valid in the industry. The tremendous alteration which is demographic the twenty-first century U.S work force has created the person concern which made the organisation non to alter their paradigms. Companies which are the best think strategically and work in dual. Watts Wacker, Jim Taylor, Howard Means, writer of airy ushers as warned that we are in go forthing in epoch where the single ability enforce their ain hereafter. Many of the companies pursue planning, where as few organisations are suitably busy with the strategic thought. It is defined as a competitory betterment for little concerns in the market. Strategic thought is defined as a careful planning in an organisation. The two chief constituents of the strategic thought were cognition of the present and point of view for the hereafter. This is called as dichotomy by the organisational schemes. Duality is followed by many of the successful companies on a regular basis. Most of the little concerns are clients of today or tomorrow sing for the hereafter effects. Features of a strategic thought which is effectual chiefly focus on keeping an attack which is long standing to appreciate the effects which are flexible. Strategic planning and vision works together, as a appropriate planning of the bases and the causes for determining the path of an organisation. Associating to employee: – The importance of associating with a culturally diverse work force can be understood by the organisational leaders who are effectual. On many occasions phrase of new engineering concerns were devalued by workers in the procedures. Follows of the mechanical parts for accomplishing the organisational aims should be sing by the leaders.Jeeffrey pfeffer writer of the human equation accepts that success of an organisation depends upon straight to the execution. Currently emergent of work force crosses the gender age race and geographics. For the first clip in the American history employees must cover with 4 coevalss of work. The leading manner which can be effectual for freshly upcoming employees fails with the employee coevals X.In little concern house ‘s leader who is effectual as to keep good relationship with his employers. Future waies: – Ignoring world-wide imperativeness and front line competitions little concern houses can accomplish success with the right approach.21st century offers the challenges for the human resources such as practical organisations, outsourcing and fiscal restraints of budgets in many federal bureaus. Small concern houses have 5 of import cardinal factors such as vision ends, strategic thought, basic accomplishments and linking with employees. You read "Business Strategy As The Direction For Organisations Business Essay" in category "Essay examples" Such jobs can be utilised as an chance for little houses to increase the rate of success. These methods are logical, practical procedure provide for the creative activity, growing and guarantee a successful little concern. Different types of positions theoretical accounts and attacks or utilised in the strategic planning. Way of developing a strategic program chiefly depends on the nature of the organisation leading, size of the organisation, civilization of the organisations, complexness of the organisation environment, expertness of contrivers etc. There are different types of strategic planning theoretical accounts which includes ends based, issues based, organic, scenario etc. The most common of the strategic planning modal is goals base planning. It chiefly focus on the vision of the organisations, schemes to accomplish the ends to work towards the machine. Issues based strategic be aftering theoretical account chiefly examines the issues which are faced by the organisation, schemes to mention those issues and action programs. Organic strategic planning starts by jointing the vision and values of the organisations and so the needed programs to make the vision while keeping to those values. Some co ntrivers chiefly prefer a peculiar attack to be aftering for illustration appreciative question. Some programs are design to one twelvemonth some to three old ages some to five to ten old ages in the hereafter. But many of the programs include merely top degree information but no action programs are available where as some programs are 5 to 8 pages long. Strategic be aftering procedure his agenda depending upon the nature of the organisation, demands of the organisations and so its external environments. Strategic planning should be followed often in an organisation. Planing might be carried out one time or twice country in a comprehensive and elaborate manner. If the organisation has been established for many old ages and has a stable place in the market topographic point so be aftering can be carried out one time a twelvemonth.Following usher lines are considered strategic planning 1. Strategic planning should be done in the initial phase of organisation when it is started. 2. Strategic planning should besides be done when developing a new division, section are presenting a major new merchandise. 3. Action programs should be updated every twelvemonth 4. Advancement of the execution of a strategic program should be supervised on a quarterly footing by the board. There are different procedures served by the strategic planning in an organisation. Purpose of the organisation should be clearly defined to set up ends and aims in a define clip frame within the capacity of the organisation for implementing. Those ends and aims are pass oning to the components of the organisation and sense of honor ship over the program has been developed.Resources of the organisation are efficaciously use by concentrating on the cardinal precedences. Advancement can be measured and mechanism is established to inform the alteration when needed. There are many other grounds which include the strategic planning helping and organisations. Strategic be aftering provides clear focal point of organisation and helps in bettering its efficiency and effectivity it besides bridges staff and board of managers increasing productiveness from addition efficiency and effectivity. It besides helps in work outing major jobs. The value of utilizing cardinal analytical tools to assist in the strategy-making procedure: – Many tools are included in the scheme doing procedure. Many analytical tools are developed to help the organisation with the planning processes, nonprofitable organisational these tools modifying the inquiries and standards with their ain particular services and markets. Environmental scan: – It is performed to garner informations to happen out replies inquiries about the present and future territory ‘s common perceptual experience is developed and it identifies strengths, failings tendencies and conditions. Environmental scans is a key for ongoing procedure for internal and external honestness and openness to altering status. There are many attacks to environmental scanning which includes service, inquirers, focal point groups and unfastened forums. SWOT analysis: – SWOT analysis is another tool used for scheme procedure. It is one of the strategic planning methods which is used to measure strengths, weeknesses, chances and menaces which are involved in a undertaking. SWOT analysis chiefly indicates the nonsubjective if a concern venture are a undertaking which helps in placing the internal and external factors which are favorable and which are non favorable to accomplish that aim. A SWOT analysis starts with a specifying a terminal province or objective it may be included in to the strategic planning theoretical account. Strengths: – Feature of a individual or a company which helps in accomplishing the aim. Failings: – Feature of the individual or a company which are harmful to accomplish the aim. Opportunities: – External conditions which are helpful to carry throughing the aim. Menaces: – External conditions which may do harm to the aim. Identifying SWOT ‘s is indispensable is of import because alternate stairss in the procedure of be aftering to accomplish the selected aim can be derived from the SWOT ‘s. Main purpose of any SWOT analysis is categorising the of import internal and external factors which play a cardinal function in finishing the aim. SWOT analysis is a aggregation of of import information is classified into two chief classs. Internal factors: – Internal strengths and failings of the organisation. External factors: – Opportunities and menaces which are subjected to the external environment organisation. Strengths and failings can be considered as the internal factors of the organisations depending on their force on the organisation aim. When strength is considered with regard to an nonsubjective it may go failings another aim. External factors may include macroeconomic affairs, technological alterations and societal cultural alterations including alterations in the market and competitory place. SWOT analysis is one of the methods of distinction with its ain failings. Importance of the single SWOTs will be exposed by the value scheme generates. Undertaking 2 As per the instance survey, MIB is a little graduated table concern which proved to be successful when it was started. Cynthia had started her concern on the strength of a individual inspiration as mentioned. She stated that she did non get down her concern with a long term program. Her vision was non strong plenty. Even though in 2001, gross revenues had exceeded $ 2.3 million, which was an addition of 12 per centum, the gross revenues were non the same in recent times. Cynthia was really much concerned about the fiscal figures. She stated that she would be interested in following new methods of gross revenues by taking the thoughts and concerns of other people in the company. Cynthia had long been the exclusive drive force behind MIB. In the 1980s, there were few makers of ace size vesture. Cynthia was really good cognizant about the defeat of big adult females whose picks were limited.She believed fat people, and particularly adult females, were oppressed and made suffering by a society that treated them as lazy. MIB offered a full line of vesture and accoutrements in four chief classs ; calling wear, insouciant athletic wear, formal wear and overclothes. Cynthia had long been the exclusive drive force behind MIB. In the 1980s, there were few makers of ace size vesture. Cynthia was really good cognizant about the defeat of big adult females whose picks were limited.She believed fat people, and particularly adult females, were oppressed and made suffering by a society that treated them as lazy. MIB offered a full line of vesture and accoutrements in four chief classs ; calling wear, insouciant athletic wear, formal wear and overclothes. The vision of MIB began on a trip up the California seashore. Cynthia stopped in a little used vesture shop and discovered a choice of drawstring army excess workss, which were 100 % cotton, inexpressive, and dyed in stylish coloring material. Cynthia ‘s retail concern, meanwhile, was booming without labour intensifier. The same gross revenues came easy but took excessively much clip in happening the right merchandise to direct. As per the Quarterly summery of all the units if MIB, we can cognize the reverse of the company. As coming to the point of the strength there are certain point that we can detect. MIB was started as a little graduated table concern with one adult male squad. It has been started as a retailed fabric shop and a became a client focused concern at all degrees supplying different types of apparels by the terminal of 2001. I would wish to categorise of the strengths and failings of the company based on below five precedences. Vision: Even though Cynthia started the company on her ain. Her chief inspiration had begun from when she was graduated from Sonoma State University. As she stated it was non plan to turn the company into a big graduated table industry. I think her vision was hapless. She stated that she learnt everything from telling, advertisement, advancing and covering with hard currency flow when she joined adult females ‘s book shop called Rising Women ‘s Books. It was merely the experience she gained from a book stored that enabled her to get down her ain concern. In 1980 she said there were really limited picks for the big adult females dressing. MIB offered a full line of vesture and accoutrements by the late 1990 ‘s.Cynthia knew from her ain experience that big adult females would love the insouciant manner, voguish colorss and blandishing tantrum. Womans of all sizes and forms bought these apparels but it was clearly the large adult females who benefited. As declared Cynthia was the lone one behind the foundation of her concern. She was unsure of her end and tried to implement her thoughts merely with a short term program. 2. Strategic planning: Cynthia started her concern with an initial sum of $ 4,500, but at this clip she did n’t see herself as an enterpriser. As per her thoughts Making It Worth was an chance to unite her personal ends with the political beliefs. She stated that if equal vesture would lend to better self esteem it would so be worthy by giving a spell. Cynthia had no experience in set uping recognition. So, she started the concern merely with the sum what she had and with one other spouse Janet. She merely realised subsequently, that it would go a great beginning of income or net income as the competition was non much. Her programs were merely limited to her accomplishments and conceptually she was still lacked with indispensable accomplishments. She felt that turning a concern would necessitate different accomplishment set than get downing one. Planning was non perfect and she merely acted as per that state of affairs. Even though, the company has a good client focused service, with initial degrees of net incomes, it has seen a sudden autumn in their retail gross revenues. This can be because of improper planning of their work flows. 3. Information thought ( accomplishments ) : As the concern was started merely by Cynthia, her accomplishment set was limited. She utilised all her accomplishments in taking the company from inexpensive frills to MIB but she stated that her accomplishment put entirely was non sufficient for MIB ‘s growing. In order to accomplish certain ends or to take a company as big graduated table it is indispensable to hold different cognition sets, and different thoughts.As Cynthia was entirely while she started her concern she was sufficient with her accomplishment set and she established a manner for her ain concern. But, in order to develop the company and achieve the marks a company needs more skilled people in different classs of the concern development program. 4. Staffing: Exhibit 6 provides a profile of distribution channels for adult females ‘s dress in the United States in 1997. Most retail merchants of big size vesture offered their goods through catalogs, and an increasing figure had set up web sites that created personal profiles for repetition clients. In 2002, there were over 2,000 makers of big size adult females ‘s vesture in the United States, and few of these lone offered vesture in supersize scope. In 2001, MIB employed five directors, a research and development squad of three members, three supervisors and a squad of 10 workers. MIB is a really labour -intensive concern and everyone works exceptionally difficult, at all degrees. But as the gross revenues were falling down Cynthia started believing of taking more appropriate people for her concern. She thought that by implementing the new thoughts, the company would see some better alterations. Cynthia believed she needed â€Å" an experient direction squad to assist pull off the hazard that ‘s needed to acquire to the following degree. † It is really of import to any company to put right people at right degrees. This makes the company more progressive and helps to accomplish the marks. As we know when the selling programs does non work out, it proves to be more expensive and dejecting. Cynthia knew that, even with her 20 old ages of experience, she still lacked indispensable accomplishments. 5. Future waies: Every Company has its future waies, like how it will be positioned in the market, what would be the end and purpose of the company. MIB besides had its long term conventional program in order to run into the estimated marks. Once the bead in the gross revenues resulted Cynthia decided to use new squad members to back up her positions and aid in the growing of MIB. I want to categorise the strengths and failings of the concern with three things, people, client and finance. Strengths aˆ? High quality direction It is really of import that we take right people for the right place. The Company ‘s growing is depended on the direction team. â€Å" How good they manage the concern that manner they get the consequences † . aˆ? Relatively stable, extremely committed Work force: As per the MIB direction squad, Cynthia was really much happy with her direction squad. She described the qualities of each individual, who were every bit talented in their corresponding Fieldss. MIB is a powerful retail trade name. It has a repute for value for money, convenience and a broad scope of merchandises all in one shop. MIB has grown well over recent old ages, and has experienced planetary enlargement for illustration its purchase of the United Kingdom based retail merchant ASDA. The company has a nucleus competency affecting its usage of information engineering to back up its international logistics system. A focussed scheme is in topographic point for human resource direction and development. Peoples are cardinal to MIB ‘s concern and it invests clip and money in developing people, and retaining a developing them. aˆ? Excellent quality in work force: It is really of import that each person works for the advancement of the company. Celebration of staff ‘s accomplishments: Low director to staff ratio Unique merchandise Location of your concern Patents, know-how, trade secrets Worker ‘s alone accomplishment set Corporate civilization, company image Quality of your merchandise Access to funding Operational efficiency Location of your concern Lack of quality and client service Poor selling and gross revenues Entree to resources Undifferentiated merchandises or services Failings Since MIB sell merchandises across many sectors ( such as vesture, nutrient, or stationary ) , it may non hold the flexibleness of some of its more focussed rivals. Training on processs non accomplishments Absence from work Nerve-racking conditions Communicationss scheme Team Leaderships and Managers excessively stretched OPPORTUNITIES THREATS aˆ? Finding a money strategian For any new investing aˆ? Improve choice techniques aˆ? Improve occupation flexiblenesss aˆ? Lack of staff in residential attention aˆ? Staff morale aˆ? Retention of societal workers aˆ? Stress for staff and Foster carers aˆ? Perception of occupation satisfaction deteriorating aˆ? Quality of societal work appliers cut downing aˆ? Keeping a qualified work force aˆ? Gender instability aˆ? Single position Opportunities and menaces are external value making ( or destructing ) factors a company can non command but emerge from either the competitory kineticss of the industry or market or from demographic, economic, political, proficient, societal, legal, or cultural factors. AnA opportunityA in the SWOT theoretical account could be for illustration: A new emerging or developing market ( niche merchandise, topographic point – new state, less competition ) Amalgamation, joint venture, or strategic confederation Market tendencies New engineerings Social alterations ( for illustration demographics ) New competition in the market, perchance with new merchandises or services Monetary value wars Economic conditions Political alterations Competitor oligopoly or monopoly Tax Handiness of resources . To take over, merge with, or organize strategic confederations with other planetary retail merchants, concentrating on specific markets such as Europe or the Greater China Region. The shops are presently merely trade in a comparatively little figure of states. Therefore there are enormous chances for future concern in spread outing consumer markets, such as China and India. New locations and shop types offer MIB chances to work market development. They diversified from big ace Centres, to local and mall-based sites. Opportunities exist for MIB to go on with its current scheme of big, ace Centres. Bing figure one means that you are the mark of competition, locally and globally. Bing a planetary retail merchant means that you are exposed to political jobs in theA states that you operate in. The cost of bring forthing many consumer merchandises tends to hold fallen because of lower fabrication costs. Manufacturing cost have fallen due to outsourcing to low-priced parts of the World. This has lead to monetary value competition, ensuing in monetary value deflation in some scopes. Intense monetary value competition is a menace. Undertaking 3 As per the analysis from, there are certain things to be considered in order MIB achieve its marks. If we check the company ‘s retail net income it is apparent that there was a sudden autumn of gross revenues. From coevals to coevals gustatory sensations of clients would alter. Companies should internally upgrade their squad and engineerings in order to vie every bit with other administrations. I think that MIB was been a Owner Manager concern which could merely accommodate few betterments. In order to decide a company from retail shop to independent good honored administration we need certain things like Human Resources, Technology, Budget and Customers good will. I would wish to explicate each of the undermentioned briefly: Human resources: Right from the Cheap frills to MIB, Cynthia was the lone individual who took attention of the concern. Cynthia ‘s accomplishments and cognition helped her to set up the thought of MIB but they were non adequate to be an profitable company. In order to set up the company as big graduated table few things would turn out indispensable. Directors, Supervisors and Staff will turn out to be the of import portion of any administration. Giving chance to more skilled people at different degrees will assist the company grow. A determination devising squad would be necessary for the company in order to obtain a fast gait profitable gross revenues. Technology: When MIB was there were really few makers of supersize vesture and the per centum of purchasers were besides less in entire. As the coevalss move on engineering is upgrading a batch go forthing people more proficient and more accurate about their sentiments. It is indispensable for any company like MIB to move consequently. Introducing different ways of advertisement like go forthing door to door catalogs, get offing and keeping information on web site helps the company to turn large. Budget: MIB was started as a really little company with really less budget. When Cynthia got an thought of widening a company she was non cognizant of the fiscal fortunes that she needs to face. In order to use new people in the company and to upgrade the engineering it is indispensable that one should raise more capital investing. How to cite Business Strategy As The Direction For Organisations Business Essay, Essay examples

Hero and Mrs. Fisher free essay sample

Heroism is a role model and someone who helps others with out personal gain or need, and they show support. Tangerine by Edward Bloor contains characters that are heroes and non-heroes. Paul Fisher is a hero while his brother, Erik, is not and Mrs. Fisher also has traits of heroism. Paul Fisher is a hero because he puts others before himself despite personal danger and because he does the right thing without need for personal gain. Paul helps people during the sinkhole incident at lake Windser Middle School. He saves other classmates and teachers from the portable classrooms, ensuring their safety (80). This shows Paul is brave and willing to protect his classmates in a horrible accident. Besides the sinkhole, Paul aids to the Cruz family during the freeze. He brings wood to Luis and chopped ice off trees, doing whatever they could to get a majority of them saved (214, 215). Paul helps the Cruz family without being asked to. We will write a custom essay sample on Hero and Mrs. Fisher or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page He lies to his mom so she would let him stay and help (210, 213). Paul was committed to save the tangerine trees of Tangerine. Since Paul is friendly to people, he always has his friends backs and he understands them. Paul defended fire fighter Wayne when Mr. Fisher strongly complained about his unprofessional appearance (22). Paul figured that Wayne didn’t deserve a complaint when he knew what he was talking about. Paul also gave Joey Costello advice to move schools and become a War Eagle (121). Joey took the advice and moved schools, and he joined their soccer team. Paul didn’t want Joey to be unhappy with his new school hours, so he suggested Tangerine High. Paul looks out for his friends and is very encouraging. Besides Paul Fisher being a hero, his mom, Mrs. Fisher, also shows examples of heroism. Mrs. Fisher is a helpful citizen and cares about everyone’s safety. She hosts a parent gathering to discuss their sons’ football practice times (54- 55). Mrs. Fisher tries changing practice times so no other player will be struck by lightning, like Mike Costello. Additionally, Mrs. Fisher shows her safety concerns to Paul’s school about his eyesight, so he could have extra help (24). She wanted to do what was best for Paul but she wanted him to have to opportunity to play soccer. When Paul moved schools, she realized the best thing would be is not to inform them about him eye conditions, that way he would be able to play soccer. Not only is Mrs. Fisher concerned about safety; she is also supportive to her family and friends. Mrs. Fisher supports Erik by attending his football games (124, 125). She encourages and cheers for Erik while he kicks. She is proud of his hard work and accomplishments. Another way Mrs. Fisher is supportive is when she shows sympathy to the Costello family. Mrs. Fisher attends Mike Costello’s funeral and explains how sorry she was and gives love and supportive gestures to acknowledge her sympathy. While Paul Fisher and Mrs. Fisher both are explained through heroism, Erik Fisher is a non-hero. Erik is very selfish and only cares about him and has success as an individual. Erik is an amazing kicker; he always bragged about his skills and was convinced that he will be a star one-day. All he cares about is football and his future of football (20, 21). Erik thought he was better than everyone else. He was happy when Mike was killed because he knew he would benefit from it (51). Furthermore, Erik is also a non-hero because he is a bully. Erik makes fun of Joey because he tried to take off his brother’s shoes when he was dead. Erik didn’t know the real reason why he did that, he just noticed it and judged him poorly (110). Erik doesn’t care about how anyone feels or how he makes him or her feel; he amuses himself with their emotional pain. Erik also makes fun of Paul about his glasses. Erik called Paul â€Å"Eclipse Boy† and made up stories about how Paul’s eyesight failed (2, 54). Heroism is someone that puts others before themselves and is a role model. Paul and Mrs. Fisher are both heroes while Erik is not. The author wrote this story because he is a middle school teacher and wanted to share a story that exampled students in ways they are heroes. There are more heroes than there are non-heroes. Edward Bloor wrote this book for middle age kids so he tried to focus on the positive sides of people. There are heroes all around you but everyone has non-hero moments, you should learn from your mistakes.

Success free essay sample

Ladies and Gentlemen, I give you none otherthan the one and only Nick A.! screamed the announcer into themicrophone, in a vain attempt to be heard over the eruption of cheers from theoverflowing auditorium. As I stood, brimming with pride, the noise grew to adeafening level. I walked slowly toward the podium, my grin growing with eachstep. The announcer shook my hand, the principal slapped me on the back, and as Istepped up to the podium, I looked up at a gigantic banner blazing forth mysuccess. It read: Nice Guy Builds Ramp! Well, it probablywould never happen that way. Nice Guy Builds Ramp! is not exactly acommon honor at an awards ceremony. The fact is I am, for the most part, a niceguy, and I did, in fact, build a ramp. And here is another fact: the truereward did not come from any ceremony but rather because I built it for someone Idid not even know, and it made a world of difference to her. We will write a custom essay sample on Success or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page She was an olderwoman, perhaps in her 70s. I never found out what was wrong with her, but she hada gigantic oxygen tank in her simply furnished living room, and she waswheelchair-bound. The whole business began when I participated in a summerprogram where teens from all over come together to do projects for people who aretoo old or poor to get them done. With other kids, I was assigned to build a rampfor this lady who had not been out of her house in seven years. At first Icould not believe I had been talked into going to a work camp, but I soon found Ihad never felt so good or had so much fun. My crew and I really bonded, with thiscommon goal of helping a woman who was only seeing the world from her window. Instantly, our group seemed to know each other. Lindsey was tall andathletic with really stretchy skin she could use to launch pencils from her knee,a talent she often demonstrated at lunch. Matt was a year younger and muchshorter, his crew cut not helping him much in the height department. We came tocall him the Handy Man because he was never without super-cargo pants that heldmore tools than seemed humanly possible. Michelle had great,super-precisely braided hair that looked as if it had taken hours to do. I wasreally impressed until one of the braids fell out. Oh, shoot, shesaid, and tied it back in. My whole world came crashing down when I realized thebraids were synthetic and that lots of girls wear them. As our workprogressed, we were so involved that we became impervious to the attacks ofpassersby who stopped to ask what we were doing and could not believe we wereworking for free. The big payoff came when our lady rolleddown the ramp for the first time. I will never forget her tears. For the firsttime in seven years she collected her mail herself. We all gave her a gigantichug. Still in a state of amazement, she invited us to visit anytime, claiming shehad plenty of soda. And as she thanked us over and over again, Ill admit it Iteared up. Building a ramp is not the only thing I was ever praised for. Iwon a bronze medal in the Rockland Final Fencing Tournament junior year, so Iknow what it is like to have people clapping when my name is called. But I alsoknow that building a ramp was a much greater success than any medal I could everwin. Success free essay sample â€Å"I don’t want to do it! I should just drop out!† I yelled. There are only two terms left, but I’ve got much better things I can be doing with my time. Like not being here. But I’m stuck here; doing work I don’t want to do, being more stressed than I need to be. I’m sitting at my desk, trying to do work, when my best friend comes in. Well, she used to be. But all the stress has really built up. I started smoking to try to deal with it, and she doesn’t approve. She begs me to stop, but I see no other way. So every night, I do my homework, find time for a smoke break, and continue. It’s better than dropping out, right? People should see things my way. I never get a break. I hate that she doesn’t want to talk to me anymore, but what else can I do? It’s 5-0, bottom of the seventh, your team is down, and you are coming up to bat. We will write a custom essay sample on Success or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page You have 2 options. You can give up before you even get up there, before the inning even starts, or you can go up there saying â€Å"I’m going to take the best three swings of my life† and give your team a chance at making that comeback. Whether or not you actually get a hit, and keep your team alive doesn’t matter as much as the decision that you make. To give up before your at bat, is to fail, is to not succeed. By giving up, and showing that you give up, you bring the rest of your team down with you. Even if it is not a team thing, more so an individual thing, the people around you will also be brought down in some way or another. To take that chance, see what you can do, that is to succeed, that determines whether your at bat was â€Å"successful† or not. Success means different things to different people. To some, it’s achieving something, becoming well-known, and the wealth that goes along with it. To others, it’s passing a test, a cla ss, an interview, a tryout, anything that applies. To me, success is simply not giving up. Doing your best, trying your hardest, when the only possible outcome seems like a lose-lose situation. Success is all about choice, and what a person does when it comes time for her to make that important choice. It’s about doing the right thing when she has the option of doing the wrong. Instinct tells her to give up, once the situation she is looking at is leaning towards a negative outcome. Her instinctual reaction is not always right, but sometimes what she decides to do is what is easiest. And what is easiest is not always what is best, it rarely is. It can be as simple as what you decide to do in that game, or as important as passing high school, passing college, or making a life for yourself. Whether or not you put in the effort, the time, or the will to achieve a goal determines what the outcome is. It’s just as easy to give up in either circumstance, though the result o f some of your actions can be life-changing. Success, to me, is the way you get through life, and handle the hard times. It’s about not bringing you morale down, or the morale of those around you. To not give up and always keep going, no matter what. Like my one teacher says, â€Å"Go, kid, go† and â€Å"never give up!† Success free essay sample When I was younger, I wished for a Porsche and a mansion. That was my idea of success. I vigorously took all the advanced classes possible and aced the exams with my mind set on going to an Ivy League school. I just knew that I would somehow become famous and be able to afford that car and the dream house. All the way through junior year, my mind was planning this idyllic future. Then in eleventh grade, multiple losses changed my na/Ove mindset. First, one of my friends died at age 16. Soon after, my great-grandmother passed, followed by my school’s assistant director and, on the same day, my beloved fourth-grade teacher. The devastation left me not knowing what to do or where to go. Death had never touched me so closely. After a long period of emptiness, it finally struck me: Life is not promised and neither is my future success. We will write a custom essay sample on Success or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page While I was going through the motions in an attempt to attain material success, I was not enjoying my daily life. I was just existing. It occurred to me that life has more purpose than acquiring possessions, which will only be yours until you can no longer keep up with the payments. Although I had often been told this, I finally understood. I realized that finding inner peace, purpose and happiness will stick with you forever and that is real success. Enjoying life’s precious quirks makes a person more successful than a wealthy person who is not content and takes everything for granted. The summer before senior year, my attitude changed drastically. Instead of memorizing facts, I began learning information. Instead of focusing on the future, I focused on today and the many blessings and successes that come with it. I still make excellent grades, but now I dedicate weeks to studying instead of cramming for exams, and I think about the future with a deeper sense of meaning. Success, just like beauty, is in the eye of the beholder. For me, being successful means truly living life each day, being thankful, and impacting the world.

Dissertation on Histone Protein Segregation The WritePass Journal

Dissertation on Histone Protein Segregation Dissertation on Histone Protein Segregation IntroductionAim Material and MethodsReagents usedExperimental ProcedureResultsDiscussionReferences Related Introduction Histone Proteins are favourably the alkaline proteins which are present in the nucleus of the eukaryotic cell. They are the basic parts responsible for wrapping and organizing DNA into chromosomes in the nucleus. They are the principal protein elements of the chromatin and acts as a spool around which the DNA winds. In the nucleus of the cell the DNA is arranged in a very dense and super coiled style to form chromosomes. This is referred to as DNA packaging. (Isenberg, 1979) The unwound DNA in the chromosomes is very long and cannot be fit in the nucleus of the cell, hence these histone proteins brings about the DNA wrapping thereby reducing the dimensions of DNA in the cell. (Alberts B et al., 2001) Histones are primarily categorized into five major types. They are H1/H5, H2A, H2B, H3 and H4. They are categorized into two classes. They include core histones with H2A, H2B and H3 and linker histones with H1 and H5. Two of each of core histones unites to form one octameric nucleosome which is the fundamental sub unit of chromatin. (Bartova et al. 2008; Bonisch et al. 2008). Hence histones proteins form the nucleosomes directly. After extraction of chromatin from the cells it is like beads on string. The string is the DNA and the beads are the nucleosomes which are roughly disc shaped. The linker histone H1 separates the nucleosome with the other nucleosomes and also helps in bringing the adjoining nucleosomes for further super coiling. The octameric nucleosome is formed by two H2A, H2B dimers and a tetramer of H3, H4 histones. These core histones are comparatively analogous in structure. However their 3D structures are relatively different. The core histones have different protein sequence. The sequence for H2A Histone is SGRGK QGGKA RAKAK SRSSR AGLQF PVGRV HRLLR KGNYS ERVGA GAPVY LAAVL EYLTA EILEL AGNAA RDNKK TRIIP RHLQL AIRND EELNK LLGRV TIAQG GVLPN IQAVL LPKKT ESHHK AKGK. (Andreas and David, 1998) The sequence for H2B Histone is PEPAK SAPAP KKGSK KAVTK AQKKD GKKRK RSRKE SYSIY VYKVL KQVHP DTGIS SKAMG IMNSF VNDIF ERIAG EASRL AHYNK RSTIT SREIQ TAVRL LLPGE LAKHA VSEGT KAVTK YTSSK. (Andreas and David, 1998) The sequence for H3 Histone is ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA CEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA. (Andreas and David, 1998) The sequence for H4 Histone is SGRGK GGKGL GKGGA KRHRK VLRDN IQGIT KPAIR RLARR GGVKR ISGLI YEETR GVLKV FLENV IRDAV TYTEH AKRKT VTAMD VVYAL KRQGR TLYGF GG. (Andreas and David, 1998) The molecular weight of the core histone proteins are measured in the Daltons. The theoretical molecular weight for H2A histone protein is 13,990.28 Daltons, for H2B histone protein is 13,788.97 Daltons, for H3 histone protein is 15,272.89 Daltons and for H4 histone protein is 11,236.15 Daltons. (Kornberg, 1977;McGhee and Felsenfeld, 1980) The histone proteins play a significant role in chromosome stabilisation, gene regulation and expression. The main functions of histone proteins are condensing the DNA strands and chromatin regulation. These histone proteins form the nucleosome and they impart a structure to which DNA is twisted and causes to fit the bulky genomes of eukaryotes inside the nucleus of the cell. (Bartova et al. 2008; Bonisch et al. 2008). The histones also undergo post translational modifications which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have very long tails projecting from the nucleosome which can be covalently altered at numerous places and the alteration includes the acetylation, ubiquitination, methylation, phosphorylation, ADP-ribosylation and citrullination.(Bartova et al. 2008; Bonisch et al. 2008). The modification of histone includes the gene regulation, DNA repair and chromosome condensation. In this manner, histones can control gene expression, cell g rowth and proliferation. (Jenuwein and Allis 2001). The separation of the histone proteins is carried out by using the 1D SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) method. In the gel electrophoresis method the charged molecules are segregated based on their physical properties like charge, mass, etc. by allowing them to pass forcedly through a gel matrix by an electric current. (Coligan et al., 2002).The proteins from the various complex protein mixtures are normally separated by this method by using the polyacrylamide gel. It is known as Polyacrylamide Gel Electrophoresis (PAGE method). In this method the main constituent is the acrylamide which is used in the preparation of the electrophoresis gels for the isolation of proteins. In order to form the cross linked polymer network, the acrylamide is combined with bisacrylamide with the polymerising agent like Ammonium Per Sulphate (APS). The polymerisation reaction is catalysed by TEMED (N,N,N,N-tetramethylenediamine) by the producing the free radicals by APS. The size of the pores and firmness of the gel matrix mainly depends on the ratio of bisacrylamide and acrylamide used and their total concentrati on. Sequentially these in turn depends up on the range of molecular weight of the proteins which has to be determined. The pore size of the gel matrix is contrary to the quantity of acrylamide used. Generally 12% of polyacrylamide gel has lesser pore size than 7% of polyacrylamide gel. Larger proteins are resolved with the gels with less amount of acrylamide and smaller proteins with more amount of acrylamide. And for the broad range of protein sizes, the special gels like Gradient gels are prepared by having less percent of acrylamide at the top/start and more percent of acrylamide at the bottom/end. The electrophoresis gels are mixed with the buffers which impart the conduction of electric current though the matrix. The solution is transferred to the gel cassette which is a thin space formed by placing two glass or plastic plates facing each other. After the gel is polymerised, the cassette is placed vertically into the electrophoresis tank containing the electrodes. The proteins are added in the wells from the top and electrophoresis is carried out during which the proteins are separated by the gel due to its sieving properties. In order to obtain the best possible resolution of the proteins a gradient gels are used. (Hames et al., 1990) There are different forms of PAGE available which are used for separation of different proteins based on different principles. They commonly include the native PAGE, SDS PAGE, 2D PAGE, etc. (Hames et al., 1990; Coligan et al., 2002). In case of the 2D PAGE (Two Dimensional Polyacrylamide Gel Electrophoresis) the proteins are isolated by isoelectric point in the first dimension and later by mass in the second dimension. This method offers the maximum resolving power for the protein analysis and it is a very significant method in proteomic research. (Hames et al., 1990) In the native PAGE, the proteins are isolated based on their size, net charge and shape. The electrophoretic migration is due to the fact that the most of the proteins possess a net negative charge in alkaline running buffers. As the negative charge density increases, the migration speeds of the protein increase. Simultaneously the frictional force of the gel matrix produces a sieving effect thereby reducing the protein movement based on their size and shape. Hence the smaller proteins face less frictional force and larger proteins face more frictional force. In this manner the various proteins are separated from a mixture. (Hames et al., 1990) In case of the SDS PAGE the gel is mixed with the buffer having the SDS (Sodium Dodecyl Sulphate). The SDS is heated with the proteins samples before electrophoresis so that the charge density of all proteins are almost equal. The SDS is an anionic detergent which denatures the proteins present in the sample and attaches strongly to the uncoiled molecule. In order to make sure that no quaternary or tertiary proteins structure remains generally a reducing agent like dithiothreitol (DTT) is also added as it ruptures the protein disulphide bonds. Hence when these samples are used in the electrophoresis, the proteins get separated based on their mass alone. In this method a set of proteins of known molecular weight is run aside the sample in the same gel cassette. They act as a reference from which the mass of the sample proteins is determined. They are called as Molecular Weight Markers. The electrophoresis is carried out by using the two gels for favourable results. They are the stacki ng gel and the resolving gel. The stacking gel is added over the top of the resolving gel. Apart from the low concentration of acrylamide in stacking gel, it has low pH and different ionic content than the resolving gel. This causes the proteins to get concentrated into a tight band during the early electrophoresis period before entering into the resolving gel. (Hames et al., 1990) Breast cancer refers to the hysterical growth of breast cells. The breast carcinoma is a malignant tumour. It occurs due to any abnormal genetic changes in the breast cell. Presently there is an increasing alarm regarding the high risk posed by various compounds with oestrogen like activity present in the environment. These various compounds includes the Phytoestrogens (e.g. genistein), food products like legumes, lentils, chickpeas, soybean, cereals, fruits, and vegetables, industrial contaminants, (e.g. bisphenol A) and polychlorinated biphenyls, organochlorine pesticides (e.g. endosulfan), etc. These substances have the ability to produce cancer mediated through estrogenic receptors. Some of these substances when treated with the MCF-7 cell line, MCF-10F (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²+), MDA-MB-231 (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²+) or MCF-10A (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²Ã¢Ë†â€™) cells, increased the growth rate of these cell lines. This 3-4 folds proliferation was due to the up regulation of all the core h istone proteins which represents the increase in the chromatin content of the cells. The degree of proliferation of the cells indicates the level of the core histone proteins and was concentration dependent. Hence this indicates that histone proteins are used as indirect markers of breast cell proliferation. Therefore in the human breast cancer the histones are up regulated and cause cell proliferation mediated by the oestrogen receptors agents. Based on this it is possible to state that the core histone proteins serve as bio markers of (ER+) human breast cancer.(Zhu et al., 2009) Aim The main aim of this experiment is to resolve the given four human recombinant protein samples (H2A, H2B, H3 and H4) by using the one dimensional SDS Poly Acrylamide Gel Electrophoresis (1D SDS – PAGE). Material and Methods Equipments used The experiment uses the Bio Rad Mini PROTEAN 3 Cell gel electrophoresis unit. It consists of the electrophoresis tank, electrode chamber with a cathode and anode, two glass plates, gel cassette assembly, comb and the connecting cables. Power source, heater, micro centrifuge, pH meter, and the other general equipments like test tubes, conical flasks, etc were used. Reagents used The various reagents used in this experiment includes the Acrylamide/bisacrylamide (30%w/v/0.8%w/v), 3.0M Tris/HCl (pH – 8.8 for resolving gel stock), 0.5M Tris/HCl (pH – 6.8 for stacking gel stock), TEMED, SDS (10%w/v), Ammonium Per Sulphate (APS – 25%w/v). The running buffer is also used as 0.025M Tris/0.192M glycine/0.1% (w/v) SDS, pH 8.3. The other reagents used are sample buffer, Pre stained molecular markers, colloidal Coomassie Blue Stain Solution, Bromophenol Blue (0.5%w/v) and the given four human recombinant proteins (1 µg/ µl) [H2A, H2B, H3.3 and H4]. Experimental Procedure The SDS PAGE method is commonly used for the examination of the proteins because of its simplicity, speed and resolving capacity. The SDS PAGE is the Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis method. The various steps performed are as follows: Preparationof Gel Cassette Sandwich Firstly select a clean Spacer Plate and Short Plate and place them over one another and set them in the casting frame and lock the pressure cams from both the sides. It should be kept in such a manner that the short plate should face the front of the frame. After locking the gel cassette assembly check that both the plates are flush at the bottom. Then place the rubber gasket at the bottom of the casting stand and place the gel cassette sandwich assembly in the casting stand in such a manner that it is perpendicular to the level surface. After complete formation of the gel cassette assembly, add water into it to make sure that no leaking takes place. Then remove the water and wipe it with the filter paper. Set the comb into the assembled gel cassette and mark the level at 1 cm below the comb teeth and remove it, the resolving gel is poured up to this level. After preparation of the resolving gel pour it up to the mark into the assembled gel cassette in a very smooth way to prevent an y air bubble formation. Allow the gel to set for 45 minutes. After the gel has set wash its surface with distilled water and wipe with the filer paper and allow drying the area in between the glass plates for a minute. Then after this prepare the stacking gel solution and pour it above the resolving gel till the top of the plates. Then immediately place the comb in the stacking gel in gel cassette to form the wells. Allow the stacking gel to set for overnight. Preparation of Gels The gels used in SDS PAGE method are the resolving gel and the stacking gel. Both the gels consists of the acrylamide/bisacrylamide, distilled water, 10% SDS, TEMED and ammonium per sulphate (APS). However the resolving gel uses 3.0M Tris/HCl (pH 8.8) and the stacking gel uses the 0.5M Tris/HCl (pH 6.8). These gels are prepared by directly mixing all the reagents. However the TEMED and APS are added only when the gel is ready to be polymerised. In this manner the gels are prepared. Electrophoresis Module Assembly After the stacking gel has set, smoothly remove the comb. Then open the cams and remove the sandwich gel cassette and place it in the electrode assembly in such a manner that the short plate faces inward of the electrode assembly. Secure the electrode assembly by closing the two levers of the frame to form the inner chamber. Set this secured inner chamber into the Mini Electrophoresis Tank. Fill the inner chamber with the running buffer, however it is not overfilled. Then add around 200 ml of the running buffer is added into the Mini Tank. Sample Preparation and Loading Four recombinant histone protein samples are used in this experiment. They are H2A, H2B, H3.3 and H4. 1 µl of each of this protein samples (1 µg/ µl) is added to 20 µl of the Sample Buffer in the Eppendorf tubes and labelled properly. They are heated in a heater set at 100 º C for two minutes and then allowed to cool to room temperature. After the sample preparation, 20 µl of each of the histone protein is loaded into the wells carefully by using the gel loading tips. The sample loading should be very smooth and the tip should not puncture the well. Load the 2 µl of the pre stained molecular marker (Page Ruler) into the other well simultaneously to ensure the movement of proteins. Gel Electrophoresis After loading the histone proteins, mini tank is covered with the lid and the electric supply is given by the cable wires. Turn on the power supply and the start the gel electrophoresis at 200 Volts for about 35 minutes until the bromophenol blue dye has drifted to 1 cm from the bottom of the gel. During this time each histone protein based on their molecular weight is moved to their ends. Staining the Gels Once the electrophoresis is completed, switch off the power supply and take out the lid. Cautiously lift the inner chamber assembly and dispense the running buffer to avoid the falling of buffer before opening the cams. Then open the cams and take out the glass cassette sandwich. Open the sandwich with the help of gel releaser and take out the gel slowly. Immerse the gel in 30 ml of Colloidal Coomassie blue stain solution for half an hour with moderate shaking. During this time the staining occurs and once it is completed the gel is de stained with water and then incubated with water at room temperature for overnight for complete de staining. After this the gel is rinsed with water for the proteins bands to be seen clearly. Results Figure 1: SDS Poly Acrylamide Gel Electrophoresis of Histone Proteins. Bands show the migration of individual histone proteins. A pre stained molecular marker is included at the side for comparison. In the present experiment four human recombinant histone proteins were given for isolation and determination. From the standard pre stained molecular marker, each of the histone protein has to be determined by correlating with its band. The Figure 1 illustrates the SDS Poly Acrylamide Gel Electrophoresis of given four recombinant histone proteins (H2A, H2B, H3.3 and H4). In the figure the last column of bands refers to the standard pre stained molecular marker (Page Ruler) and the other four bands represents the respective histone proteins. The values beside the molecular marker indicate the molecular weight in kDa. Hence with reference to this standard values, the four given histone protein samples has to be determined. In case of the band of H2A histone protein, it is nearly 13,990 Da as it is below 15 kDa. Similarly in case of the band of H2B histone protein, it is nearly 13,789 Da. And in case of H3.3 histone protein, the band is clearly at the top of all the bands indicating it has the highest value and it is slightly above 15, so it may be around 15,273 Da. In the fourth histone protein H4, the band is at the far end when compared to the other three bands. However it is slightly above 10. Hence it may be around 11,236 Da. Therefore the molecular weights of the histone proteins were identified. However the standard molecular weight for the H2A is 13,990.28 Da, for H2B is 13,788.97 Da, for H3.3 is 15,272.89 Da and for H4 is 11,236.15 Da. (Kornberg, 1977;McGhee and Felsenfeld, 1980) Discussion The separation of proteins is mainly performed by the gel electrophoresis method. There are different types of gel electrophoresis methods used to separate different types of proteins. However in the present experiment the isolation of histone proteins is mainly carried out by 1D SDS PAGE method. This method is a one dimensional method and it separates the proteins principally by their molecular weight. It makes use of the ionic detergent Sodium Dodecyl Sulphate (SDS). The poly acrylamide gel is mixed with SDS because it denatures the proteins and binds to them to make them approximately evenly negatively charged. This is done with the aid of heating and generally a reducing agent like dithiotheritol (DTT) in order to break the protein disulphide bonds. This removes the risks of presence of any tertiary or quaternary proteins. As a result when electric current is passed through a power supply, all the SDS bound proteins of the sample will drift in the gel matrix to the anode, thereby separating the proteins based on their molecular weight alone. The proteins with low molecular weight moves fast through the gel compared to the proteins with high molecular weight due to the sieving effect of the gel matrix. Therefore in this experiment a set of four human recombinant histone proteins (H2A, H2B H3.3 and H4) were given to separate them based on their molecular weight. Hence these protein samples were taken and were subjected to the SDS PAGE method. In this method firstly the gel cassette assembly was formed by using the two glass plates (Spacer Plate and Short Plate) in a proper manner such that the short plate is facing front of the frame. After assembling the gel cassette it was filled by the gels which were prepared just before use. Firstly the resolving gel was poured and after it got set for about 45 minutes, the stacking gel was poured and comb was placed in it and it was allowed to set for overnight. The pouring of gels was carried out very carefully and slowly in order to prevent any air bubble formation. The appropriately set gel with the well defined wells is selected and gel cassette assembly was set in the electrode unit in the Mini Electrophoresis Tank. The running buffer was added properly and the sample histone proteins were loaded very cautiously. Before loading the protein samples, they were heated for about two minutes. Along with the samples, the pre stained molecular marker was also loaded. And the power supply was given and the electrophoresis was carried out for 35 minutes at 200V. During this time the bromophenol blue dye moves to 1cm from the bottom of the gel. After this the power supply was switch off and very carefully the gel was removed from the gel cassette sandwich assembly and was dipped in the staining solution like Colloidal Coomassie blue stain solution for about half an hour with occasional shaking. After staining, the gel is removed and de stained with distilled water by incubating it for overnight. Then it was rinsed properly to visualise the bands for each of the histone proteins. The pre stained molecular marker of known molecular weight was also run beside the given histone samples in the same gel. It acts as a standard or reference. For all the given four human recombinant histone proteins the bands lie in between the 10 and 15 kDa as per the marker. In situation of H2A histone protein, the band obtained is just below the 15 kDa band in the standard. Hence its molecular weight is around 14 kDa. In case of the H2B histone protein, the band is obtained also below 15 kDa but a little lower than H2A, hence it should be less than 14 and should be around 13.7 kDa. In case of H3.3 histone protein, the band is just above the 15 kDa and it should be around 15.3 kDa and for the H4 histone protein the band is quite low and just above the 10 kDa band; hence it should be around 11.3 kDa. The data obtained in this experiment is fairly correct as compared to the standard theoretical data. The theoretical molecular weight for H2A histone protein is 13,990.28 Daltons, for H 2B histone protein is 13,788.97 Daltons, for H3 histone protein is 15,272.89 Daltons and for H4 histone protein is 11,236.15 Daltons. (Kornberg, 1977;McGhee and Felsenfeld, 1980). Hence the results obtained in this experiment are reasonably acceptable and can be used for further studies. If the mixture of these histone proteins were need to be separated by the same method (SDS PAGE), then the expected result would not be accurate and proper. And the band for the individual histone protein may not be clear. It is because all the given four human recombinant histone proteins have approximately equal and very close molecular weights. And the values for all the four histone proteins lie between 10 to 16 kDa. Hence the bands would be very close to each other. If any flaws or any possible inaccuracy occurs during the experiment, then there is a possibility that the bands may be wrong. Hence the results would be wrong. Therefore there is very less possibility that the results would be accurate and also for the correct identification of the bands. However, if the experiment is carried out perfectly, then in such case the order of bands would be H4, H2B, H2A and H3.3 in the increasing order of their molecular weights. It is resolved with the help of the standard molecular mar ker which acts as a reference. Therefore, if mixture of these four histone proteins were to be separated by this method very accurately, then the results may be correct. However, for the separation of this mixture of four histone proteins another laboratory method is available. In this method, the groups (H2A, H2B) and (H3, H4) are separated first by using NaCl and hydroxylapatite chromatography. (Richard and Gary, 1979) The H2A and H2B group are the slight lysine rich group and H3 and H4 is the arginine rich group. This method was introduced by Van der Westhuyzen and Von Holt. In this method, firstly the individual groups were separated. However, the given mixture was treated with 0.63 M NaCl and 0.1 M potassium phosphate before the mixture sample is loaded on to the hydroxylapatite. This is to remove any contamination with the other linker histone proteins like H1 and H5. Then it was subjected to chromatography to obtain the core histone groups (H2A, H2B); (H3, H4). After this the samples obtained are subjected to SDS PAGE method for further separation. Finally the core histones were separated. (Richard and Gary, 1979) References Ahmad, K. and Henikoff, S. (2002). Histone H3 variants specify modes of chromatin assembly Proc. Natl. Acad. Sci. U. S. A. 99 Suppl 4, 16477-16484. Andreas, D.B. and David, L. (1998 Oxford University Press). Nucleic acids research. Histone Sequence Database: new histone fold family Vol. 26, No. 1, 372-375. Bartova, E. Krejci, J. Harnicarova, A. Galiova, G. and Kozubek, S. (2008). Histone modifications and nuclear architecture: A review J. Histochem. Cytochem. 56, 711-721. Bonisch, C. Nieratschker, S.M. Orfanos, N.K. and Hake, S.B. (2008). Chromatin proteomics and epigenetic regulatory circuits. Expert Rev Proteomics.5, 105–119. Coligan, J.E., et al. , Eds. (2002). Electrophoresis, In Current Protocols in Protein Science, John Wiley and Sons, Inc. New York. 10.0.1-10.4.36. Hames, B.D. and Rickwood, D. Eds. (1990) Gel Electrophoresis of Proteins: a Practical Approach, 2nd ed. Oxford University Press, New York. Isenberg, I. (1979). Histones. Annu. Rev. Biochem. 48, 159-191. Jenuwein, T, and Allis, C.D. (2001). Translating the histone code. 293. 1074–1080. Kerenyi, L. and Gallyas, F. (1973). Errors in quantitative estimations on agar electrophoresis using silver stain Clin. Chim. Acta 47, 425-436. Kornberg, R. D. (1977). Structure of chromatin Annu. Rev. Biochem. 46, 931-954. Marino-Ramirez, L. Jordan, I. K. and Landsman, D. (2006). Multiple independent evolutionary solutions to core histone gene regulation Genome Biol. 7, R122. McGhee, J. D. and Felsenfeld, G. (1980). Nucleosome structure Annu. Rev. Biochem. 49, 1115-1156. Richard, H.S. and Gary, F. (February 1979). Nucleic acids research, oxford journals. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite Volume 6 Number 2, 689-696. Rà ¼chel, R. Steere, R. L. and Erbe, E. F. (1978). Transmission-electron microscopic observations of freeze-etched polyacrylamide gels? Journal of Chromatography A 166, 563-575. Shapiro, A. L. Vinuela, E. and Maizel, J. V.,Jr. (1967). Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels Biochem. Biophys. Res. Commun. 28, 815-820. vanWert, J. M. Wolfe, S. A. and Grimes, S. R. (2008; 2008). Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes J. Cell. Biochem. 104, 1087-1101. Weber, K. and Osborn, M. (1969). The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis J. Biol. Chem. 244, 4406-4412. Zhu, Z. Edwards, R. J. and Boobis, A. R. (2009). Increased expression of histone proteins during estrogen-mediated cell proliferation Environ. Health Perspect. 117, 928-934. Version 112,113, 114,115 and 116. UniProtKB/Swiss-prot release 2010_04 P16104

NAFTA Opposing View Essay Example | Topics and Well Written Essays - 500 words

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How global warming affects sea levels Research Paper

How global warming affects sea levels - Research Paper Example Essentially, the greenhouse effect functions in the following manner. When sunlight pierces the atmosphere and hits the earth’s surface, not all of the sun’s solar energy is absorbed. Approximately a third of it is reflected back into space. Specific atmospheric gases serve in much the same way as does the glass of a greenhouse, thus the terminology. These gases allow sunlight to penetrate then trap some of the solar energy which heats the earth (Breuer, 1980). It is a delicate balance and because these greenhouse gases have been artificially augmented by man-made sources, more build up in the atmosphere has occurred thus trapping more of the sun’s energy and reflecting less back in to space. This occurrence is causing the earth to warm. The rising levels of carbon dioxide (CO2) in the atmosphere are becoming increasingly disconcerting. â€Å"The concentrations of CO2 in the air around 1860 before the effects of industrialization were felt, is assumed to have been about 290 parts per million (ppm). In the hundred years and more since then, the concentration has increased by about 10 percent.† (Breuer, 1980, p. 67). Eighty percent of the world’s population accounts for just 35 percent of CO2 emissions while the United States and Soviet Union combined are responsible for generating half. Worldwide, â€Å"carbon dioxide emissions are increasing by four percent a year.† (Miller, 1990, p. 450). Motor vehicles are a major cause of air pollution as is fuel burned for the heating of homes and powering industry along with the toxins emitted from stacks at coal-burning power plants. â€Å"Vehicles produce high levels of carbon monoxides (CO) and a major source of hydrocarbons (HC) and nitrogen oxides (NO x), whereas, fuel combustion in stationary sources is the dominant source of sulfur dioxide (SO2)† (Breuer, 1980, p. 70). If the balance between the CO2 levels in the ocean and atmosphere is disturbed by interjecting increasing

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Suppose you were selected for special position - Essay Example This take however may be challenged, especially when a not sought for assignment is offered, and there are no chances of refusal. If I get myself in such a fix, then it would be required that I apply my course and every single thing I have learnt in the process in dealing with the issue at hand. For me, my course and especially physics would play a central role. Ethics Moral responsibility plays a central role in any given assignment. Regardless of the specific field of training, this is a fundamental component of one self, not only in the field of employment, but also in any given context of human interactions. The various education fields have different ethics applicable to them (Perl 213). This encompasses physics among all other identifiable fields of study. In this regard, taking into account the codes of ethics applicable to me, given the course I have taken, I am morally responsible to take on assignments offered to me. This does not only imply personal benefit, but also the w elfare of the entire society; the entire company included. Training The education system in place takes care of individual interests, not only in academic terms, but also in every identifiable aspect of life. Training that makes me handle not sought for assignments encompass class work, related practical work as well as outside-class exposure. This is an input factor that has been realized along the line of study, such that it becomes integral to whatever goals and objectives pursued during and after successful completion of studies. Personal diversity and dynamism Personal growth and development is neither rigid nor fixed to specific grounds of capability. Physics is a unique subject that captures changes in a very specific way (Perl 288). Most of the worldwide development, innovativeness and inventions borrow heavily from physics and related subjects. The diversity and dynamism characteristic of this subject and the course in general should be manifested through the learners. Succ essful completion of this course implied that all requirements prior to this course have been met. In this regard, applying the concepts, theories and models presented in the course should not amount to any difficulty. The important thing to note is that the specific theories and concepts presented in the course are not likely to be the same as those that will be encountered in the actual line of work. Experiencing a different scenario from that I am already used to is a challenge. However, I take challenge as a factor of determination, where personal diversity and dynamism is based upon. This means that even if I land on a not sought for assignment, there are no regrets nor are there doubts that I can actually handle it. Being open and broad minded is the crucial factor that determines personal diversity and change over time. Fixing my career to the specifics of the course does not imply learning for me. This course has taught me that knowledge is a procedural attainment through wh ich a known phenomenon leads to an unknown, and that unknown, another phenomenon of knowing is created. In this regard, the new technology meant to produce electricity may not be known to any employees of the company, but it is known to some other party not present in the company. This brings in the fundamental point to consider. That is, there exists information about this technology and how it works to achieve the desired purpose. As a Lead

Oral Communication Grammar Mistakes

Oral Communication Grammar Mistakes One of the most common problems during the oral communication is that people dont know grammar rules and how to use them properly. There are people who know a lot of words, but when they want to make a sentence they arent able to do it because they lack knowledge of grammar. There are also a few more problems that affect normal communication that will be mentioned in this paper. This study will deal with problem of people making so many mistakes during oral communication, and with possible resolution of that problem. Key words: oral communication, grammar, mistakes 1. INTRODUCTION Good communication is very important. It is important in terms of the individuals success personally, in their personal relationships, and it is important for their professional succes. In most cases employers are looking for employees who posses good communication skills, and by that, they want them to be able to read and write effectively, to be able to orally communicate with others effectively, to participate in teams. Effective communication skills are one of the major predictors of upward mobility in the organization, that is, people who have good communication skills are more likely to get promoted, to get raises, to end up getting positions that they want. People with good communication skills are not only more effective in informing others and persuading others, theyre also more effective at getting support from others and acquiring information from others, theyre acquiring emotional support from others and getting others to believe in them and what theyre doing. These skill s are extremely important in any aspect of professional life. In the personal sector, good communication skills matter so much in terms of the quality of family relationships, the relationship with close partners. Good communication is part of being a good parent, communicating effectively with your child, with your neighbors, and with others in your community. 2. COMMUNICATION Proper definition of communication could be that it is the activity of conveying information through the exchange of thoughts, messages, or information, as by speech, visuals, signals,writing, or behaviour. Oral communication is purposeful presentation designed to increace knowledge, to foster understanding, or to promote change in the liseners attitudes, values, beliefs and behaviours. Communication requires a sender, a message, and a receiver, although the receiver doesnt need to be present or aware of the senders intent to communicate at the time of communication; thus communication can occur across vast distances in time and space. Communication requires that the communicating parties share an area of communicative commonality. The communication process is complete once the receiver has understood the message of the sender. Good communication can be considered as creative activity which takes different forms depending from many factors, including the characteristics of the communicator (perception of self, attitude towards language learning, the level of self expectation in language use, perception of others), the process of making the meaning and responding(linguistic components the purpose of interaction) and the context in which the interaction happens. Scientist Lynn Harter said about communication: à ¢Ã¢â€š ¬Ã… ¾Communication is an integral part of a liber al studies education because what communication helps students to focus on how they use symbolic resources at hand to create and maintain relationships, to participate as a citizen in the public sphere, whether thats attending a city council meeting and exercising their voice about a local initiative or petition. Or maybe its being involved on a board of directors for a local non-profit organization and being the person whos responsible for communicating with various stakeholders of that organization.  [1]   Types of oral communication are: Intrapersonal communication This kind of communication refers to a conversation that people hold with themself in some situations-for example, when a person needs to make an important decision. This kind of communication can be considered as the process of thinking. Interpersonal communication This kind of communication occurs between several people. Interpersonal communication can occur face to face and also through electronic channels like video-conferencing, chat rooms, e-mail,twitter. Small group communication This type of communication occurs in a small group of people. Small group communication helps people to interact with others. On this way peopleteach to exchange ideas,solve problems and share experiences with each other. Public communication or public speaking This typeof communication occurs between a speaker and an audience. Public speaking serves for the speaker to give important informations to audience. It is impotant to have effective public speaking so that the speaker can influence his audience when he communicates with them. Mass communication This type of oral communication is sent out from a source to many receivers all aroun the world. It occurs through media, through internet and blogs. Corporate communication This type of communication occurs between members of an organisation, within that organisation. Good corporate communication has a positive impact on morale, commitment and productivity in corporations. Intercultural communication Intercultural communication can be defined as communication between people of different cultures and ethnicity. The world is increasingly becoming a global village and every country has people of various ethnicities. Thus, it is important to note differences in the communication practices of different cultures if intercultural harmony and understanding is to be maintained. 3. BUSINESS COMMUNICATION To the success of any organization despite its size, geographical location, good business communication is extremely important. Business communication is linked with the internal culture and external image of any organization. This type of communication determines what is communicated, by whom and at what level. Effective communication can help the organization in achieving its goal by informing, persuading and building good will within both the internal environment and the external environment. Communication, internal and external, takes place in a nonverbal and verbal manner: through gestures, expressions, meetings and through listening,speaking and writing. Global environment is quickly changing and if organizations want to survive and prosper they need to change constanttly their way communication process are formed and delivered. Good communication skills can be helpful to peoplein their careers in various ways: It can build selfconfidence, make useful contracts, enable you to help and lead others. Most successful people recognize that communication was vital to their job success.Most of them said that communication skills were more important than the major subject they had studied in college. By improving communication one can help not only himself but the entire organization. That is because as an employee one forms a link in the organizations information chain. Peoples ability to receive, evaluate, use and pass on information gathered from inside and outside the organization affects your companys effectiveness. Managers rely on such information from their employees to take suitable and necessary action. By keeping employees informed about the happenings , by involving them in decision-making, and getting feedback from them regarding their opinions, attitudes and suggestions, managers can raise employee morale. As a result, employees are more willing to assume responsibility and support managements efforts. Communication scholar said: There are three things that I believe are very important to communicating in organizations. The first thing I would say is be aware of the context. If you dont understand the context, you dont know what something means. So what does it mean, for example, if a CEO is earning a thousand times more money than the lowest paid employee in an organization? Well, to an outside watchdog group, that could be corporate greed. To financial analysts on Wall Street, that could be good management. So you have to understand the context if youre going to understand the meaning of communication. The second thing I would suggest that people think about in terms of communicating in organizations is that communication creates relationships. A lot of people think all communication is about getting your point across, making sure that youre clear in your communication. I take a bit of a different view. Communication creates the relationships that you have with people. Theres that old saying, sticks and stones may break my bones but words will never harm me. It is a terrible, terrible axiom because the words we do use can harm people because they create different relationships. So how you talk does matter. The third thing I would simply say is that people need to be curious about communication. I think one of the biggest problems people run into in organizations when they communicate is they think they know what the other person means. Many times we act on false assumptions. You need to get curious about what people are saying and ask questions like, well, what do you mean by that? and where do you think this is going to take us? and just a number of different questions to sort out what it is that people are meaning because we come from different kinds of experiences, different backgrounds and we need to really understand what a person means by the words that they use.  [2]   4. DIFFERENCES BETWEEN ORAL AND WRITTEN COMMUNICATION Communication requires the transfer of informations from one person to another, and this kind of transfer of informations is dynamic. Writing is a static process of transfer of informations. The written language can be more precise. The choice of written words can be done with greater deliberation and thought. Writer can rewrite his text .The reader can read the text as he wishes. He can do it quickly, slowly or the reader can stop thinking in order to think about the informations that he has just read. The reader also has a possibility of re-reading the text. Speeches should be precise. There needs to be a lot of preparations and compresion for oral communication to be precise. When a word is once spoken it cant be retracted. The speaker can only apoligize for a mistake. One can read from a written text and can achieve with it the same degree of verbal precision as written communication. In most cases audience finds speech-reading uninteresting and boring. Oral communication is considered to be effective in expressing meaning to an audience. During the speech a few signals are available to a speaker: gestures, intonation,ninflection, volume, pitch, pauses, movement, visual cues as appearence The speaker has more control over the informations that the listener will hear than the writer has over what the reader will read. Reading an audience is a systematic and cumulative endeavor unavailable to the writer. As one speaks, the audience provides its own visual cues about whether it is finding the argument coherent, comprehensible, or interesting. Speakers should avoid focusing on single individuals within an audience. 5. COMMUNICATION COMPETENCE The notion of communicative competence was introduced by dell Hymes in the late 1960s. Children grow up in the midst of verbal interactions in which they need to pay attention not only to grammatical form (the order of words and their inflection) but also to the implications, for themselves and other participants, of what is said. To the extent to which linguistic research is concerned with the association between forms and meanings, it should recognize that any linguistic expression is associated with and often reproduces a set of attitudes, values, and beliefs about the world. To be a fluent speaker means to be able to recognize and exploit such attitudes, values and beliefs. Furthermore, language is often only one of the codes used for communication. Speakers complement, refine, and sometimes neutralize or even contradict the meaning of words they use by relying on gestures and eye gaze, and by the use of tools and other material resources around them. The notion of communicative competence was also meant to encourage the study of different codes and modes of communicating within and across situations. Although the term competence echoes Chomskys use of the same word, the addition of the adjective communicative broadens the object of inquiry. Furthermore, while Chomskys notion of competence refers to an abstract set of (typically unconscious) rules-based on an innate language faculty and independent of actual usage-Hymes notion of competence refers to the knowledge necessary for adequately communicating in real life situations. Hence the attention to what speakers actually do with language that is implicit in the notion of communicative competence is not only a methodological but also a theoretical concern. That is, it not only allows us to start from what people do with language in order to infer what they need to know, but alsorecognizes that knowledge of language is gained from participation in actual communicative events, implying the simultaneous relian ce on a number of semiotic resources and the collaboration of other participants. In this sense, Hymes notion of competence is not separate from the notion of performance the use of language as it is in Chompskys model. The introduction of the notion of communicative competence was consistent with Hymes earlier call for a new interdisciplinary field, the etnography of communication (sometimes called the etnography of speaking). This field is dedicated to the empirical study of patterns of speaking in different communities around the world. Theoretically, it draw from a number of traditions, most notably Roman JakobsonS functionalism as represented in his speech event model, from which Hymes derived his notion of communicative event, which is a unit of analysis conceived for the study of communicative competence. In This framework, speaker competence is no longer defined exclusively in terms of their knowledge of grammatical rules, but is also defined in terms of the range of communicative events in which they can participate. Such a range defines persons repertoire within a given community. 6. MOST USUAL MISTAKES IN ORAL COMMUNICATION Commuincators in the process of communication are influenced by several inputs and a deficiency in the quality of any of these inputs can prevent successful communication. According to Adler and Rodman(2006), communicators personal and social background, perception of self and others and verbal and non-verbal language skills are the main inputs influencing the process of communication. Communicators personal background factors such as age, motivation, literacy, and social class make the language learning experience of all individuals unique.  [3]   There are a few factors which can affect oral communication: Language difficulties The use of jargon Accent Physical condition affecting speach or hearing Environmental condition Grammar problems If the persons dont speak the same language fluently, normal conversation is hard to expect. Evidently they will have troubles in understanding each other. Here are a few english words whic are most commonly misspronounced: Archipelago Arctic Accessory Athlete Candidate Chaos Clothes Dilate Height Heinous Hierarchy Illinois Interpret Miniature mischievous Orient Old-fashioned Picture precipitation Drowned Preventive Pronunciation Prostate Jewelry Library Medievel Accent is also one of the factors that can strongly affect the communication between people. If two persons have different accents their communication may not be succesful. Their differences in speach may create misunderstandings. Physical condition affecting speach or hearing is one of the important factors that can influence normal communicaton. If persons who are communicating have a speech or hearing impairment the way that they communicate may have to be adapted. One of the factors by which oral communication is affected is enviromental condition. If persons are communicating in noisy conditions there might be the possibility that one of them miss the important information. In this conditions normal communication is very hard to achieve. Jargon is one of the factors that can affect communication. Especially in working situations people use words and phrases that mean something to them and their colleagues in their workplace or industry but which could be a foreign language to others. Grammar problem is important factor which affects communication. If people lack knowledge of grammar it is hard for them to create sentence, and it is hard to have normal communication in this conditions. There are few most common mistakes that people usually make. Here are the examples of a few gramaticaly incorrect sentences: The man which works here is from Brazil. Ana is married with a doctor. John must to call Isabel immadiately. Although it was snowing,but we had a good time. I like very much chocolate. He can to walk. Where I can find this person? (Where can I find this person?) I have been here since five months. The room is not enough big. (The room is not big enough.) There is nine boys in the bus. We didnt see nobody. He leaves in 6:00 am. Do you like a cup of coffee? Nick should not to drink alcohol 7. IMPROVEMENT OF ORALCOMMUNICATION No matter how good one knows the language, during the oral communication people make mistakes. These mistakes happen from different reasons. Even though oral communication is an effective way of receiving your message across to your listeners, it can also create misunderstanding, misinterpretation and confusion. This happens because each person has his own way of interpreting even when you speak the same language as your listener. There are a few ways to avoid this problems and to improve oral communication. One of the ways to improve oral communication is reading (books and other scriptures). If the person wants to avoid the problem of misunderstanding then it would be good to use simple words that can be clearly understood. Also in order to improve oral skills it is recomandable for a person to be a good listener, because effective communication includes speaking and active listening. If a person listens carefully , what the speaker is talking about, he improves his understanding. If a person wants his listeneres to understand the message it is good to tell a story, joke or read a quote. Jokes usually help people to relax, and in that way their mind will be more open to listen to a speaker. The way in which the story is told can affect the thinking of the listeners. Asking the right questions is also helpful in order to improve communication. A à ¢Ã¢â€š ¬Ã… ¾Why questions gives a person a lot of reasons, excuses, understanding and explanations. à ¢Ã¢â€š ¬Ã… ¾How questions set someones brain thinking for a solution, useful information and a strategy. By asking questions one can create a positive debate and interaction that will benefit all involved. If a speaker wants his speech to be good he should think and prepare before he speaks.. Misunderstanding can occur if a speaker exppresses himself without thinking and allows his emotions to take over. It is good for a speaker to create thoughts in his mind first and then to tell them aloud. Communication can be considered effective if a speaker reduces the use of verbal pauses. If a speaker often stops his sentence and adds à ¢Ã¢â€š ¬Ã… ¾ah, à ¢Ã¢â€š ¬Ã… ¾um, à ¢Ã¢â€š ¬Ã… ¾well it can be very irritating to his listeners. To solve this problemm its good for a speaker to record his own voice and in that way he will be aware of all the mistakes he does during the speech. In this we way the speaker can improve his style of speaking. To have an effective commnication speaker should use words and phrases with care. Speaker shouldnt allow himself that people assume what he is trying to say. Speaker should speak with specifity, and words like never, ever, all, or always need to be avoided. One of the characteristics that speaker should posses is honesty, because if the speaker is promising something to people that is not possible it will break down any trust that the speaker has developed. It is also recomandable for a speaker to relax while speaking. It is important that one doesnt speak in hurry, because that could create impresion that the speaker is confused. Eye contact is also important. If the speaker is facing a crowd he shouldnt look at one person for more than 5 seconds. 8.CONCLUSION From this paper we could learn the most common mistakes people do during oral communication. Most people think that communication is about transferring informations clearly. Regardless of the context, of who youre talking to and of the situation, you need to be clear in your communication. I think thats a misperception because there are times at which being ambiguous can be very effective in your communication. I think one of the reasons that this is a misperception-that we need to be clear-is that our dominant way of thinking about communication is that its about getting your point across, or what some people call transferring information. I think we need to think about communication differently and change the perception of what communication is, that communication is about creating relationships, whether thats creating romantic relationships, whether thats creating relationships in a team, in the workplace, say between an employee and his or her boss, or whether thats creating relationships among nations or countries. It is good to think about communication as a process of creating relationship. So how we communicate creates different things. I think one of the biggest misperceptions is thinking that clear communication is good communication. And I think the reason we think that is because we have a model of communication that says that communication is about transferring information. I think that misperception goes away if we change our perception to one in which communication creates relationships.