Success free essay sample

Ladies and Gentlemen, I give you none otherthan the one and only Nick A.! screamed the announcer into themicrophone, in a vain attempt to be heard over the eruption of cheers from theoverflowing auditorium. As I stood, brimming with pride, the noise grew to adeafening level. I walked slowly toward the podium, my grin growing with eachstep. The announcer shook my hand, the principal slapped me on the back, and as Istepped up to the podium, I looked up at a gigantic banner blazing forth mysuccess. It read: Nice Guy Builds Ramp! Well, it probablywould never happen that way. Nice Guy Builds Ramp! is not exactly acommon honor at an awards ceremony. The fact is I am, for the most part, a niceguy, and I did, in fact, build a ramp. And here is another fact: the truereward did not come from any ceremony but rather because I built it for someone Idid not even know, and it made a world of difference to her. We will write a custom essay sample on Success or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page She was an olderwoman, perhaps in her 70s. I never found out what was wrong with her, but she hada gigantic oxygen tank in her simply furnished living room, and she waswheelchair-bound. The whole business began when I participated in a summerprogram where teens from all over come together to do projects for people who aretoo old or poor to get them done. With other kids, I was assigned to build a rampfor this lady who had not been out of her house in seven years. At first Icould not believe I had been talked into going to a work camp, but I soon found Ihad never felt so good or had so much fun. My crew and I really bonded, with thiscommon goal of helping a woman who was only seeing the world from her window. Instantly, our group seemed to know each other. Lindsey was tall andathletic with really stretchy skin she could use to launch pencils from her knee,a talent she often demonstrated at lunch. Matt was a year younger and muchshorter, his crew cut not helping him much in the height department. We came tocall him the Handy Man because he was never without super-cargo pants that heldmore tools than seemed humanly possible. Michelle had great,super-precisely braided hair that looked as if it had taken hours to do. I wasreally impressed until one of the braids fell out. Oh, shoot, shesaid, and tied it back in. My whole world came crashing down when I realized thebraids were synthetic and that lots of girls wear them. As our workprogressed, we were so involved that we became impervious to the attacks ofpassersby who stopped to ask what we were doing and could not believe we wereworking for free. The big payoff came when our lady rolleddown the ramp for the first time. I will never forget her tears. For the firsttime in seven years she collected her mail herself. We all gave her a gigantichug. Still in a state of amazement, she invited us to visit anytime, claiming shehad plenty of soda. And as she thanked us over and over again, Ill admit it Iteared up. Building a ramp is not the only thing I was ever praised for. Iwon a bronze medal in the Rockland Final Fencing Tournament junior year, so Iknow what it is like to have people clapping when my name is called. But I alsoknow that building a ramp was a much greater success than any medal I could everwin. Success free essay sample â€Å"I don’t want to do it! I should just drop out!† I yelled. There are only two terms left, but I’ve got much better things I can be doing with my time. Like not being here. But I’m stuck here; doing work I don’t want to do, being more stressed than I need to be. I’m sitting at my desk, trying to do work, when my best friend comes in. Well, she used to be. But all the stress has really built up. I started smoking to try to deal with it, and she doesn’t approve. She begs me to stop, but I see no other way. So every night, I do my homework, find time for a smoke break, and continue. It’s better than dropping out, right? People should see things my way. I never get a break. I hate that she doesn’t want to talk to me anymore, but what else can I do? It’s 5-0, bottom of the seventh, your team is down, and you are coming up to bat. We will write a custom essay sample on Success or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page You have 2 options. You can give up before you even get up there, before the inning even starts, or you can go up there saying â€Å"I’m going to take the best three swings of my life† and give your team a chance at making that comeback. Whether or not you actually get a hit, and keep your team alive doesn’t matter as much as the decision that you make. To give up before your at bat, is to fail, is to not succeed. By giving up, and showing that you give up, you bring the rest of your team down with you. Even if it is not a team thing, more so an individual thing, the people around you will also be brought down in some way or another. To take that chance, see what you can do, that is to succeed, that determines whether your at bat was â€Å"successful† or not. Success means different things to different people. To some, it’s achieving something, becoming well-known, and the wealth that goes along with it. To others, it’s passing a test, a cla ss, an interview, a tryout, anything that applies. To me, success is simply not giving up. Doing your best, trying your hardest, when the only possible outcome seems like a lose-lose situation. Success is all about choice, and what a person does when it comes time for her to make that important choice. It’s about doing the right thing when she has the option of doing the wrong. Instinct tells her to give up, once the situation she is looking at is leaning towards a negative outcome. Her instinctual reaction is not always right, but sometimes what she decides to do is what is easiest. And what is easiest is not always what is best, it rarely is. It can be as simple as what you decide to do in that game, or as important as passing high school, passing college, or making a life for yourself. Whether or not you put in the effort, the time, or the will to achieve a goal determines what the outcome is. It’s just as easy to give up in either circumstance, though the result o f some of your actions can be life-changing. Success, to me, is the way you get through life, and handle the hard times. It’s about not bringing you morale down, or the morale of those around you. To not give up and always keep going, no matter what. Like my one teacher says, â€Å"Go, kid, go† and â€Å"never give up!† Success free essay sample When I was younger, I wished for a Porsche and a mansion. That was my idea of success. I vigorously took all the advanced classes possible and aced the exams with my mind set on going to an Ivy League school. I just knew that I would somehow become famous and be able to afford that car and the dream house. All the way through junior year, my mind was planning this idyllic future. Then in eleventh grade, multiple losses changed my na/Ove mindset. First, one of my friends died at age 16. Soon after, my great-grandmother passed, followed by my school’s assistant director and, on the same day, my beloved fourth-grade teacher. The devastation left me not knowing what to do or where to go. Death had never touched me so closely. After a long period of emptiness, it finally struck me: Life is not promised and neither is my future success. We will write a custom essay sample on Success or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page While I was going through the motions in an attempt to attain material success, I was not enjoying my daily life. I was just existing. It occurred to me that life has more purpose than acquiring possessions, which will only be yours until you can no longer keep up with the payments. Although I had often been told this, I finally understood. I realized that finding inner peace, purpose and happiness will stick with you forever and that is real success. Enjoying life’s precious quirks makes a person more successful than a wealthy person who is not content and takes everything for granted. The summer before senior year, my attitude changed drastically. Instead of memorizing facts, I began learning information. Instead of focusing on the future, I focused on today and the many blessings and successes that come with it. I still make excellent grades, but now I dedicate weeks to studying instead of cramming for exams, and I think about the future with a deeper sense of meaning. Success, just like beauty, is in the eye of the beholder. For me, being successful means truly living life each day, being thankful, and impacting the world.

Dissertation on Histone Protein Segregation The WritePass Journal

Dissertation on Histone Protein Segregation Dissertation on Histone Protein Segregation IntroductionAim Material and MethodsReagents usedExperimental ProcedureResultsDiscussionReferences Related Introduction Histone Proteins are favourably the alkaline proteins which are present in the nucleus of the eukaryotic cell. They are the basic parts responsible for wrapping and organizing DNA into chromosomes in the nucleus. They are the principal protein elements of the chromatin and acts as a spool around which the DNA winds. In the nucleus of the cell the DNA is arranged in a very dense and super coiled style to form chromosomes. This is referred to as DNA packaging. (Isenberg, 1979) The unwound DNA in the chromosomes is very long and cannot be fit in the nucleus of the cell, hence these histone proteins brings about the DNA wrapping thereby reducing the dimensions of DNA in the cell. (Alberts B et al., 2001) Histones are primarily categorized into five major types. They are H1/H5, H2A, H2B, H3 and H4. They are categorized into two classes. They include core histones with H2A, H2B and H3 and linker histones with H1 and H5. Two of each of core histones unites to form one octameric nucleosome which is the fundamental sub unit of chromatin. (Bartova et al. 2008; Bonisch et al. 2008). Hence histones proteins form the nucleosomes directly. After extraction of chromatin from the cells it is like beads on string. The string is the DNA and the beads are the nucleosomes which are roughly disc shaped. The linker histone H1 separates the nucleosome with the other nucleosomes and also helps in bringing the adjoining nucleosomes for further super coiling. The octameric nucleosome is formed by two H2A, H2B dimers and a tetramer of H3, H4 histones. These core histones are comparatively analogous in structure. However their 3D structures are relatively different. The core histones have different protein sequence. The sequence for H2A Histone is SGRGK QGGKA RAKAK SRSSR AGLQF PVGRV HRLLR KGNYS ERVGA GAPVY LAAVL EYLTA EILEL AGNAA RDNKK TRIIP RHLQL AIRND EELNK LLGRV TIAQG GVLPN IQAVL LPKKT ESHHK AKGK. (Andreas and David, 1998) The sequence for H2B Histone is PEPAK SAPAP KKGSK KAVTK AQKKD GKKRK RSRKE SYSIY VYKVL KQVHP DTGIS SKAMG IMNSF VNDIF ERIAG EASRL AHYNK RSTIT SREIQ TAVRL LLPGE LAKHA VSEGT KAVTK YTSSK. (Andreas and David, 1998) The sequence for H3 Histone is ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA CEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA. (Andreas and David, 1998) The sequence for H4 Histone is SGRGK GGKGL GKGGA KRHRK VLRDN IQGIT KPAIR RLARR GGVKR ISGLI YEETR GVLKV FLENV IRDAV TYTEH AKRKT VTAMD VVYAL KRQGR TLYGF GG. (Andreas and David, 1998) The molecular weight of the core histone proteins are measured in the Daltons. The theoretical molecular weight for H2A histone protein is 13,990.28 Daltons, for H2B histone protein is 13,788.97 Daltons, for H3 histone protein is 15,272.89 Daltons and for H4 histone protein is 11,236.15 Daltons. (Kornberg, 1977;McGhee and Felsenfeld, 1980) The histone proteins play a significant role in chromosome stabilisation, gene regulation and expression. The main functions of histone proteins are condensing the DNA strands and chromatin regulation. These histone proteins form the nucleosome and they impart a structure to which DNA is twisted and causes to fit the bulky genomes of eukaryotes inside the nucleus of the cell. (Bartova et al. 2008; Bonisch et al. 2008). The histones also undergo post translational modifications which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have very long tails projecting from the nucleosome which can be covalently altered at numerous places and the alteration includes the acetylation, ubiquitination, methylation, phosphorylation, ADP-ribosylation and citrullination.(Bartova et al. 2008; Bonisch et al. 2008). The modification of histone includes the gene regulation, DNA repair and chromosome condensation. In this manner, histones can control gene expression, cell g rowth and proliferation. (Jenuwein and Allis 2001). The separation of the histone proteins is carried out by using the 1D SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) method. In the gel electrophoresis method the charged molecules are segregated based on their physical properties like charge, mass, etc. by allowing them to pass forcedly through a gel matrix by an electric current. (Coligan et al., 2002).The proteins from the various complex protein mixtures are normally separated by this method by using the polyacrylamide gel. It is known as Polyacrylamide Gel Electrophoresis (PAGE method). In this method the main constituent is the acrylamide which is used in the preparation of the electrophoresis gels for the isolation of proteins. In order to form the cross linked polymer network, the acrylamide is combined with bisacrylamide with the polymerising agent like Ammonium Per Sulphate (APS). The polymerisation reaction is catalysed by TEMED (N,N,N,N-tetramethylenediamine) by the producing the free radicals by APS. The size of the pores and firmness of the gel matrix mainly depends on the ratio of bisacrylamide and acrylamide used and their total concentrati on. Sequentially these in turn depends up on the range of molecular weight of the proteins which has to be determined. The pore size of the gel matrix is contrary to the quantity of acrylamide used. Generally 12% of polyacrylamide gel has lesser pore size than 7% of polyacrylamide gel. Larger proteins are resolved with the gels with less amount of acrylamide and smaller proteins with more amount of acrylamide. And for the broad range of protein sizes, the special gels like Gradient gels are prepared by having less percent of acrylamide at the top/start and more percent of acrylamide at the bottom/end. The electrophoresis gels are mixed with the buffers which impart the conduction of electric current though the matrix. The solution is transferred to the gel cassette which is a thin space formed by placing two glass or plastic plates facing each other. After the gel is polymerised, the cassette is placed vertically into the electrophoresis tank containing the electrodes. The proteins are added in the wells from the top and electrophoresis is carried out during which the proteins are separated by the gel due to its sieving properties. In order to obtain the best possible resolution of the proteins a gradient gels are used. (Hames et al., 1990) There are different forms of PAGE available which are used for separation of different proteins based on different principles. They commonly include the native PAGE, SDS PAGE, 2D PAGE, etc. (Hames et al., 1990; Coligan et al., 2002). In case of the 2D PAGE (Two Dimensional Polyacrylamide Gel Electrophoresis) the proteins are isolated by isoelectric point in the first dimension and later by mass in the second dimension. This method offers the maximum resolving power for the protein analysis and it is a very significant method in proteomic research. (Hames et al., 1990) In the native PAGE, the proteins are isolated based on their size, net charge and shape. The electrophoretic migration is due to the fact that the most of the proteins possess a net negative charge in alkaline running buffers. As the negative charge density increases, the migration speeds of the protein increase. Simultaneously the frictional force of the gel matrix produces a sieving effect thereby reducing the protein movement based on their size and shape. Hence the smaller proteins face less frictional force and larger proteins face more frictional force. In this manner the various proteins are separated from a mixture. (Hames et al., 1990) In case of the SDS PAGE the gel is mixed with the buffer having the SDS (Sodium Dodecyl Sulphate). The SDS is heated with the proteins samples before electrophoresis so that the charge density of all proteins are almost equal. The SDS is an anionic detergent which denatures the proteins present in the sample and attaches strongly to the uncoiled molecule. In order to make sure that no quaternary or tertiary proteins structure remains generally a reducing agent like dithiothreitol (DTT) is also added as it ruptures the protein disulphide bonds. Hence when these samples are used in the electrophoresis, the proteins get separated based on their mass alone. In this method a set of proteins of known molecular weight is run aside the sample in the same gel cassette. They act as a reference from which the mass of the sample proteins is determined. They are called as Molecular Weight Markers. The electrophoresis is carried out by using the two gels for favourable results. They are the stacki ng gel and the resolving gel. The stacking gel is added over the top of the resolving gel. Apart from the low concentration of acrylamide in stacking gel, it has low pH and different ionic content than the resolving gel. This causes the proteins to get concentrated into a tight band during the early electrophoresis period before entering into the resolving gel. (Hames et al., 1990) Breast cancer refers to the hysterical growth of breast cells. The breast carcinoma is a malignant tumour. It occurs due to any abnormal genetic changes in the breast cell. Presently there is an increasing alarm regarding the high risk posed by various compounds with oestrogen like activity present in the environment. These various compounds includes the Phytoestrogens (e.g. genistein), food products like legumes, lentils, chickpeas, soybean, cereals, fruits, and vegetables, industrial contaminants, (e.g. bisphenol A) and polychlorinated biphenyls, organochlorine pesticides (e.g. endosulfan), etc. These substances have the ability to produce cancer mediated through estrogenic receptors. Some of these substances when treated with the MCF-7 cell line, MCF-10F (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²+), MDA-MB-231 (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²+) or MCF-10A (ERÃŽ ±Ã¢Ë†â€™/ERÃŽ ²Ã¢Ë†â€™) cells, increased the growth rate of these cell lines. This 3-4 folds proliferation was due to the up regulation of all the core h istone proteins which represents the increase in the chromatin content of the cells. The degree of proliferation of the cells indicates the level of the core histone proteins and was concentration dependent. Hence this indicates that histone proteins are used as indirect markers of breast cell proliferation. Therefore in the human breast cancer the histones are up regulated and cause cell proliferation mediated by the oestrogen receptors agents. Based on this it is possible to state that the core histone proteins serve as bio markers of (ER+) human breast cancer.(Zhu et al., 2009) Aim The main aim of this experiment is to resolve the given four human recombinant protein samples (H2A, H2B, H3 and H4) by using the one dimensional SDS Poly Acrylamide Gel Electrophoresis (1D SDS – PAGE). Material and Methods Equipments used The experiment uses the Bio Rad Mini PROTEAN 3 Cell gel electrophoresis unit. It consists of the electrophoresis tank, electrode chamber with a cathode and anode, two glass plates, gel cassette assembly, comb and the connecting cables. Power source, heater, micro centrifuge, pH meter, and the other general equipments like test tubes, conical flasks, etc were used. Reagents used The various reagents used in this experiment includes the Acrylamide/bisacrylamide (30%w/v/0.8%w/v), 3.0M Tris/HCl (pH – 8.8 for resolving gel stock), 0.5M Tris/HCl (pH – 6.8 for stacking gel stock), TEMED, SDS (10%w/v), Ammonium Per Sulphate (APS – 25%w/v). The running buffer is also used as 0.025M Tris/0.192M glycine/0.1% (w/v) SDS, pH 8.3. The other reagents used are sample buffer, Pre stained molecular markers, colloidal Coomassie Blue Stain Solution, Bromophenol Blue (0.5%w/v) and the given four human recombinant proteins (1 µg/ µl) [H2A, H2B, H3.3 and H4]. Experimental Procedure The SDS PAGE method is commonly used for the examination of the proteins because of its simplicity, speed and resolving capacity. The SDS PAGE is the Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis method. The various steps performed are as follows: Preparationof Gel Cassette Sandwich Firstly select a clean Spacer Plate and Short Plate and place them over one another and set them in the casting frame and lock the pressure cams from both the sides. It should be kept in such a manner that the short plate should face the front of the frame. After locking the gel cassette assembly check that both the plates are flush at the bottom. Then place the rubber gasket at the bottom of the casting stand and place the gel cassette sandwich assembly in the casting stand in such a manner that it is perpendicular to the level surface. After complete formation of the gel cassette assembly, add water into it to make sure that no leaking takes place. Then remove the water and wipe it with the filter paper. Set the comb into the assembled gel cassette and mark the level at 1 cm below the comb teeth and remove it, the resolving gel is poured up to this level. After preparation of the resolving gel pour it up to the mark into the assembled gel cassette in a very smooth way to prevent an y air bubble formation. Allow the gel to set for 45 minutes. After the gel has set wash its surface with distilled water and wipe with the filer paper and allow drying the area in between the glass plates for a minute. Then after this prepare the stacking gel solution and pour it above the resolving gel till the top of the plates. Then immediately place the comb in the stacking gel in gel cassette to form the wells. Allow the stacking gel to set for overnight. Preparation of Gels The gels used in SDS PAGE method are the resolving gel and the stacking gel. Both the gels consists of the acrylamide/bisacrylamide, distilled water, 10% SDS, TEMED and ammonium per sulphate (APS). However the resolving gel uses 3.0M Tris/HCl (pH 8.8) and the stacking gel uses the 0.5M Tris/HCl (pH 6.8). These gels are prepared by directly mixing all the reagents. However the TEMED and APS are added only when the gel is ready to be polymerised. In this manner the gels are prepared. Electrophoresis Module Assembly After the stacking gel has set, smoothly remove the comb. Then open the cams and remove the sandwich gel cassette and place it in the electrode assembly in such a manner that the short plate faces inward of the electrode assembly. Secure the electrode assembly by closing the two levers of the frame to form the inner chamber. Set this secured inner chamber into the Mini Electrophoresis Tank. Fill the inner chamber with the running buffer, however it is not overfilled. Then add around 200 ml of the running buffer is added into the Mini Tank. Sample Preparation and Loading Four recombinant histone protein samples are used in this experiment. They are H2A, H2B, H3.3 and H4. 1 µl of each of this protein samples (1 µg/ µl) is added to 20 µl of the Sample Buffer in the Eppendorf tubes and labelled properly. They are heated in a heater set at 100 º C for two minutes and then allowed to cool to room temperature. After the sample preparation, 20 µl of each of the histone protein is loaded into the wells carefully by using the gel loading tips. The sample loading should be very smooth and the tip should not puncture the well. Load the 2 µl of the pre stained molecular marker (Page Ruler) into the other well simultaneously to ensure the movement of proteins. Gel Electrophoresis After loading the histone proteins, mini tank is covered with the lid and the electric supply is given by the cable wires. Turn on the power supply and the start the gel electrophoresis at 200 Volts for about 35 minutes until the bromophenol blue dye has drifted to 1 cm from the bottom of the gel. During this time each histone protein based on their molecular weight is moved to their ends. Staining the Gels Once the electrophoresis is completed, switch off the power supply and take out the lid. Cautiously lift the inner chamber assembly and dispense the running buffer to avoid the falling of buffer before opening the cams. Then open the cams and take out the glass cassette sandwich. Open the sandwich with the help of gel releaser and take out the gel slowly. Immerse the gel in 30 ml of Colloidal Coomassie blue stain solution for half an hour with moderate shaking. During this time the staining occurs and once it is completed the gel is de stained with water and then incubated with water at room temperature for overnight for complete de staining. After this the gel is rinsed with water for the proteins bands to be seen clearly. Results Figure 1: SDS Poly Acrylamide Gel Electrophoresis of Histone Proteins. Bands show the migration of individual histone proteins. A pre stained molecular marker is included at the side for comparison. In the present experiment four human recombinant histone proteins were given for isolation and determination. From the standard pre stained molecular marker, each of the histone protein has to be determined by correlating with its band. The Figure 1 illustrates the SDS Poly Acrylamide Gel Electrophoresis of given four recombinant histone proteins (H2A, H2B, H3.3 and H4). In the figure the last column of bands refers to the standard pre stained molecular marker (Page Ruler) and the other four bands represents the respective histone proteins. The values beside the molecular marker indicate the molecular weight in kDa. Hence with reference to this standard values, the four given histone protein samples has to be determined. In case of the band of H2A histone protein, it is nearly 13,990 Da as it is below 15 kDa. Similarly in case of the band of H2B histone protein, it is nearly 13,789 Da. And in case of H3.3 histone protein, the band is clearly at the top of all the bands indicating it has the highest value and it is slightly above 15, so it may be around 15,273 Da. In the fourth histone protein H4, the band is at the far end when compared to the other three bands. However it is slightly above 10. Hence it may be around 11,236 Da. Therefore the molecular weights of the histone proteins were identified. However the standard molecular weight for the H2A is 13,990.28 Da, for H2B is 13,788.97 Da, for H3.3 is 15,272.89 Da and for H4 is 11,236.15 Da. (Kornberg, 1977;McGhee and Felsenfeld, 1980) Discussion The separation of proteins is mainly performed by the gel electrophoresis method. There are different types of gel electrophoresis methods used to separate different types of proteins. However in the present experiment the isolation of histone proteins is mainly carried out by 1D SDS PAGE method. This method is a one dimensional method and it separates the proteins principally by their molecular weight. It makes use of the ionic detergent Sodium Dodecyl Sulphate (SDS). The poly acrylamide gel is mixed with SDS because it denatures the proteins and binds to them to make them approximately evenly negatively charged. This is done with the aid of heating and generally a reducing agent like dithiotheritol (DTT) in order to break the protein disulphide bonds. This removes the risks of presence of any tertiary or quaternary proteins. As a result when electric current is passed through a power supply, all the SDS bound proteins of the sample will drift in the gel matrix to the anode, thereby separating the proteins based on their molecular weight alone. The proteins with low molecular weight moves fast through the gel compared to the proteins with high molecular weight due to the sieving effect of the gel matrix. Therefore in this experiment a set of four human recombinant histone proteins (H2A, H2B H3.3 and H4) were given to separate them based on their molecular weight. Hence these protein samples were taken and were subjected to the SDS PAGE method. In this method firstly the gel cassette assembly was formed by using the two glass plates (Spacer Plate and Short Plate) in a proper manner such that the short plate is facing front of the frame. After assembling the gel cassette it was filled by the gels which were prepared just before use. Firstly the resolving gel was poured and after it got set for about 45 minutes, the stacking gel was poured and comb was placed in it and it was allowed to set for overnight. The pouring of gels was carried out very carefully and slowly in order to prevent any air bubble formation. The appropriately set gel with the well defined wells is selected and gel cassette assembly was set in the electrode unit in the Mini Electrophoresis Tank. The running buffer was added properly and the sample histone proteins were loaded very cautiously. Before loading the protein samples, they were heated for about two minutes. Along with the samples, the pre stained molecular marker was also loaded. And the power supply was given and the electrophoresis was carried out for 35 minutes at 200V. During this time the bromophenol blue dye moves to 1cm from the bottom of the gel. After this the power supply was switch off and very carefully the gel was removed from the gel cassette sandwich assembly and was dipped in the staining solution like Colloidal Coomassie blue stain solution for about half an hour with occasional shaking. After staining, the gel is removed and de stained with distilled water by incubating it for overnight. Then it was rinsed properly to visualise the bands for each of the histone proteins. The pre stained molecular marker of known molecular weight was also run beside the given histone samples in the same gel. It acts as a standard or reference. For all the given four human recombinant histone proteins the bands lie in between the 10 and 15 kDa as per the marker. In situation of H2A histone protein, the band obtained is just below the 15 kDa band in the standard. Hence its molecular weight is around 14 kDa. In case of the H2B histone protein, the band is obtained also below 15 kDa but a little lower than H2A, hence it should be less than 14 and should be around 13.7 kDa. In case of H3.3 histone protein, the band is just above the 15 kDa and it should be around 15.3 kDa and for the H4 histone protein the band is quite low and just above the 10 kDa band; hence it should be around 11.3 kDa. The data obtained in this experiment is fairly correct as compared to the standard theoretical data. The theoretical molecular weight for H2A histone protein is 13,990.28 Daltons, for H 2B histone protein is 13,788.97 Daltons, for H3 histone protein is 15,272.89 Daltons and for H4 histone protein is 11,236.15 Daltons. (Kornberg, 1977;McGhee and Felsenfeld, 1980). Hence the results obtained in this experiment are reasonably acceptable and can be used for further studies. If the mixture of these histone proteins were need to be separated by the same method (SDS PAGE), then the expected result would not be accurate and proper. And the band for the individual histone protein may not be clear. It is because all the given four human recombinant histone proteins have approximately equal and very close molecular weights. And the values for all the four histone proteins lie between 10 to 16 kDa. Hence the bands would be very close to each other. If any flaws or any possible inaccuracy occurs during the experiment, then there is a possibility that the bands may be wrong. Hence the results would be wrong. Therefore there is very less possibility that the results would be accurate and also for the correct identification of the bands. However, if the experiment is carried out perfectly, then in such case the order of bands would be H4, H2B, H2A and H3.3 in the increasing order of their molecular weights. It is resolved with the help of the standard molecular mar ker which acts as a reference. Therefore, if mixture of these four histone proteins were to be separated by this method very accurately, then the results may be correct. However, for the separation of this mixture of four histone proteins another laboratory method is available. In this method, the groups (H2A, H2B) and (H3, H4) are separated first by using NaCl and hydroxylapatite chromatography. (Richard and Gary, 1979) The H2A and H2B group are the slight lysine rich group and H3 and H4 is the arginine rich group. This method was introduced by Van der Westhuyzen and Von Holt. In this method, firstly the individual groups were separated. However, the given mixture was treated with 0.63 M NaCl and 0.1 M potassium phosphate before the mixture sample is loaded on to the hydroxylapatite. This is to remove any contamination with the other linker histone proteins like H1 and H5. Then it was subjected to chromatography to obtain the core histone groups (H2A, H2B); (H3, H4). After this the samples obtained are subjected to SDS PAGE method for further separation. Finally the core histones were separated. (Richard and Gary, 1979) References Ahmad, K. and Henikoff, S. (2002). Histone H3 variants specify modes of chromatin assembly Proc. Natl. Acad. Sci. U. S. A. 99 Suppl 4, 16477-16484. Andreas, D.B. and David, L. (1998 Oxford University Press). Nucleic acids research. Histone Sequence Database: new histone fold family Vol. 26, No. 1, 372-375. Bartova, E. Krejci, J. Harnicarova, A. Galiova, G. and Kozubek, S. (2008). Histone modifications and nuclear architecture: A review J. Histochem. Cytochem. 56, 711-721. Bonisch, C. Nieratschker, S.M. Orfanos, N.K. and Hake, S.B. (2008). Chromatin proteomics and epigenetic regulatory circuits. Expert Rev Proteomics.5, 105–119. Coligan, J.E., et al. , Eds. (2002). Electrophoresis, In Current Protocols in Protein Science, John Wiley and Sons, Inc. New York. 10.0.1-10.4.36. Hames, B.D. and Rickwood, D. Eds. (1990) Gel Electrophoresis of Proteins: a Practical Approach, 2nd ed. Oxford University Press, New York. Isenberg, I. (1979). Histones. Annu. Rev. Biochem. 48, 159-191. Jenuwein, T, and Allis, C.D. (2001). Translating the histone code. 293. 1074–1080. Kerenyi, L. and Gallyas, F. (1973). Errors in quantitative estimations on agar electrophoresis using silver stain Clin. Chim. Acta 47, 425-436. Kornberg, R. D. (1977). Structure of chromatin Annu. Rev. Biochem. 46, 931-954. Marino-Ramirez, L. Jordan, I. K. and Landsman, D. (2006). Multiple independent evolutionary solutions to core histone gene regulation Genome Biol. 7, R122. McGhee, J. D. and Felsenfeld, G. (1980). Nucleosome structure Annu. Rev. Biochem. 49, 1115-1156. Richard, H.S. and Gary, F. (February 1979). Nucleic acids research, oxford journals. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite Volume 6 Number 2, 689-696. Rà ¼chel, R. Steere, R. L. and Erbe, E. F. (1978). Transmission-electron microscopic observations of freeze-etched polyacrylamide gels? Journal of Chromatography A 166, 563-575. Shapiro, A. L. Vinuela, E. and Maizel, J. V.,Jr. (1967). Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels Biochem. Biophys. Res. Commun. 28, 815-820. vanWert, J. M. Wolfe, S. A. and Grimes, S. R. (2008; 2008). Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes J. Cell. Biochem. 104, 1087-1101. Weber, K. and Osborn, M. (1969). The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis J. Biol. Chem. 244, 4406-4412. Zhu, Z. Edwards, R. J. and Boobis, A. R. (2009). Increased expression of histone proteins during estrogen-mediated cell proliferation Environ. Health Perspect. 117, 928-934. Version 112,113, 114,115 and 116. UniProtKB/Swiss-prot release 2010_04 P16104

NAFTA Opposing View Essay Example | Topics and Well Written Essays - 500 words

NAFTA Opposing View - Essay Example Its nice to paint such a bleak picture, however, what the report fails to state are the overwhelmingly positive aspects of NAFTA especially in regards to job creation. Losing 766,000 jobs from the manufacturing sector compared to creating millions of new jobs in the service sector, is a comparison the report failed to make. Jobs in the service sector normally pay a higher average salary, thereby affording individuals an improved lifestyle. Many of the individuals that complain about those lost jobs in the manufacturing sector are people who have not seized the opportunity to educate (or re-educate) oneself in the more lucrative industries which America's society seems to be moving towards. The report also states that NAFTA has not helped anyone but the companies, and the investors in those companies that use NAFTA to import and export goods and services to those three countries. Only those people who work for companies that advantageously use NAFTA, benefit from that usage Are those companies comprised of robots There are no people that take their earnings and go spend them (helping the economy) from those companies coffers There aren't other companies that supply or sell services to those import/export companies The people that work for those other companies work for free Those questions are answered by those companies that continue to suppl

How global warming affects sea levels Research Paper

How global warming affects sea levels - Research Paper Example Essentially, the greenhouse effect functions in the following manner. When sunlight pierces the atmosphere and hits the earth’s surface, not all of the sun’s solar energy is absorbed. Approximately a third of it is reflected back into space. Specific atmospheric gases serve in much the same way as does the glass of a greenhouse, thus the terminology. These gases allow sunlight to penetrate then trap some of the solar energy which heats the earth (Breuer, 1980). It is a delicate balance and because these greenhouse gases have been artificially augmented by man-made sources, more build up in the atmosphere has occurred thus trapping more of the sun’s energy and reflecting less back in to space. This occurrence is causing the earth to warm. The rising levels of carbon dioxide (CO2) in the atmosphere are becoming increasingly disconcerting. â€Å"The concentrations of CO2 in the air around 1860 before the effects of industrialization were felt, is assumed to have been about 290 parts per million (ppm). In the hundred years and more since then, the concentration has increased by about 10 percent.† (Breuer, 1980, p. 67). Eighty percent of the world’s population accounts for just 35 percent of CO2 emissions while the United States and Soviet Union combined are responsible for generating half. Worldwide, â€Å"carbon dioxide emissions are increasing by four percent a year.† (Miller, 1990, p. 450). Motor vehicles are a major cause of air pollution as is fuel burned for the heating of homes and powering industry along with the toxins emitted from stacks at coal-burning power plants. â€Å"Vehicles produce high levels of carbon monoxides (CO) and a major source of hydrocarbons (HC) and nitrogen oxides (NO x), whereas, fuel combustion in stationary sources is the dominant source of sulfur dioxide (SO2)† (Breuer, 1980, p. 70). If the balance between the CO2 levels in the ocean and atmosphere is disturbed by interjecting increasing

Suppose you were selected for special position Essay

Suppose you were selected for special position - Essay Example This take however may be challenged, especially when a not sought for assignment is offered, and there are no chances of refusal. If I get myself in such a fix, then it would be required that I apply my course and every single thing I have learnt in the process in dealing with the issue at hand. For me, my course and especially physics would play a central role. Ethics Moral responsibility plays a central role in any given assignment. Regardless of the specific field of training, this is a fundamental component of one self, not only in the field of employment, but also in any given context of human interactions. The various education fields have different ethics applicable to them (Perl 213). This encompasses physics among all other identifiable fields of study. In this regard, taking into account the codes of ethics applicable to me, given the course I have taken, I am morally responsible to take on assignments offered to me. This does not only imply personal benefit, but also the w elfare of the entire society; the entire company included. Training The education system in place takes care of individual interests, not only in academic terms, but also in every identifiable aspect of life. Training that makes me handle not sought for assignments encompass class work, related practical work as well as outside-class exposure. This is an input factor that has been realized along the line of study, such that it becomes integral to whatever goals and objectives pursued during and after successful completion of studies. Personal diversity and dynamism Personal growth and development is neither rigid nor fixed to specific grounds of capability. Physics is a unique subject that captures changes in a very specific way (Perl 288). Most of the worldwide development, innovativeness and inventions borrow heavily from physics and related subjects. The diversity and dynamism characteristic of this subject and the course in general should be manifested through the learners. Succ essful completion of this course implied that all requirements prior to this course have been met. In this regard, applying the concepts, theories and models presented in the course should not amount to any difficulty. The important thing to note is that the specific theories and concepts presented in the course are not likely to be the same as those that will be encountered in the actual line of work. Experiencing a different scenario from that I am already used to is a challenge. However, I take challenge as a factor of determination, where personal diversity and dynamism is based upon. This means that even if I land on a not sought for assignment, there are no regrets nor are there doubts that I can actually handle it. Being open and broad minded is the crucial factor that determines personal diversity and change over time. Fixing my career to the specifics of the course does not imply learning for me. This course has taught me that knowledge is a procedural attainment through wh ich a known phenomenon leads to an unknown, and that unknown, another phenomenon of knowing is created. In this regard, the new technology meant to produce electricity may not be known to any employees of the company, but it is known to some other party not present in the company. This brings in the fundamental point to consider. That is, there exists information about this technology and how it works to achieve the desired purpose. As a Lead

Oral Communication Grammar Mistakes

Oral Communication Grammar Mistakes One of the most common problems during the oral communication is that people dont know grammar rules and how to use them properly. There are people who know a lot of words, but when they want to make a sentence they arent able to do it because they lack knowledge of grammar. There are also a few more problems that affect normal communication that will be mentioned in this paper. This study will deal with problem of people making so many mistakes during oral communication, and with possible resolution of that problem. Key words: oral communication, grammar, mistakes 1. INTRODUCTION Good communication is very important. It is important in terms of the individuals success personally, in their personal relationships, and it is important for their professional succes. In most cases employers are looking for employees who posses good communication skills, and by that, they want them to be able to read and write effectively, to be able to orally communicate with others effectively, to participate in teams. Effective communication skills are one of the major predictors of upward mobility in the organization, that is, people who have good communication skills are more likely to get promoted, to get raises, to end up getting positions that they want. People with good communication skills are not only more effective in informing others and persuading others, theyre also more effective at getting support from others and acquiring information from others, theyre acquiring emotional support from others and getting others to believe in them and what theyre doing. These skill s are extremely important in any aspect of professional life. In the personal sector, good communication skills matter so much in terms of the quality of family relationships, the relationship with close partners. Good communication is part of being a good parent, communicating effectively with your child, with your neighbors, and with others in your community. 2. COMMUNICATION Proper definition of communication could be that it is the activity of conveying information through the exchange of thoughts, messages, or information, as by speech, visuals, signals,writing, or behaviour. Oral communication is purposeful presentation designed to increace knowledge, to foster understanding, or to promote change in the liseners attitudes, values, beliefs and behaviours. Communication requires a sender, a message, and a receiver, although the receiver doesnt need to be present or aware of the senders intent to communicate at the time of communication; thus communication can occur across vast distances in time and space. Communication requires that the communicating parties share an area of communicative commonality. The communication process is complete once the receiver has understood the message of the sender. Good communication can be considered as creative activity which takes different forms depending from many factors, including the characteristics of the communicator (perception of self, attitude towards language learning, the level of self expectation in language use, perception of others), the process of making the meaning and responding(linguistic components the purpose of interaction) and the context in which the interaction happens. Scientist Lynn Harter said about communication: à ¢Ã¢â€š ¬Ã… ¾Communication is an integral part of a liber al studies education because what communication helps students to focus on how they use symbolic resources at hand to create and maintain relationships, to participate as a citizen in the public sphere, whether thats attending a city council meeting and exercising their voice about a local initiative or petition. Or maybe its being involved on a board of directors for a local non-profit organization and being the person whos responsible for communicating with various stakeholders of that organization.  [1]   Types of oral communication are: Intrapersonal communication This kind of communication refers to a conversation that people hold with themself in some situations-for example, when a person needs to make an important decision. This kind of communication can be considered as the process of thinking. Interpersonal communication This kind of communication occurs between several people. Interpersonal communication can occur face to face and also through electronic channels like video-conferencing, chat rooms, e-mail,twitter. Small group communication This type of communication occurs in a small group of people. Small group communication helps people to interact with others. On this way peopleteach to exchange ideas,solve problems and share experiences with each other. Public communication or public speaking This typeof communication occurs between a speaker and an audience. Public speaking serves for the speaker to give important informations to audience. It is impotant to have effective public speaking so that the speaker can influence his audience when he communicates with them. Mass communication This type of oral communication is sent out from a source to many receivers all aroun the world. It occurs through media, through internet and blogs. Corporate communication This type of communication occurs between members of an organisation, within that organisation. Good corporate communication has a positive impact on morale, commitment and productivity in corporations. Intercultural communication Intercultural communication can be defined as communication between people of different cultures and ethnicity. The world is increasingly becoming a global village and every country has people of various ethnicities. Thus, it is important to note differences in the communication practices of different cultures if intercultural harmony and understanding is to be maintained. 3. BUSINESS COMMUNICATION To the success of any organization despite its size, geographical location, good business communication is extremely important. Business communication is linked with the internal culture and external image of any organization. This type of communication determines what is communicated, by whom and at what level. Effective communication can help the organization in achieving its goal by informing, persuading and building good will within both the internal environment and the external environment. Communication, internal and external, takes place in a nonverbal and verbal manner: through gestures, expressions, meetings and through listening,speaking and writing. Global environment is quickly changing and if organizations want to survive and prosper they need to change constanttly their way communication process are formed and delivered. Good communication skills can be helpful to peoplein their careers in various ways: It can build selfconfidence, make useful contracts, enable you to help and lead others. Most successful people recognize that communication was vital to their job success.Most of them said that communication skills were more important than the major subject they had studied in college. By improving communication one can help not only himself but the entire organization. That is because as an employee one forms a link in the organizations information chain. Peoples ability to receive, evaluate, use and pass on information gathered from inside and outside the organization affects your companys effectiveness. Managers rely on such information from their employees to take suitable and necessary action. By keeping employees informed about the happenings , by involving them in decision-making, and getting feedback from them regarding their opinions, attitudes and suggestions, managers can raise employee morale. As a result, employees are more willing to assume responsibility and support managements efforts. Communication scholar said: There are three things that I believe are very important to communicating in organizations. The first thing I would say is be aware of the context. If you dont understand the context, you dont know what something means. So what does it mean, for example, if a CEO is earning a thousand times more money than the lowest paid employee in an organization? Well, to an outside watchdog group, that could be corporate greed. To financial analysts on Wall Street, that could be good management. So you have to understand the context if youre going to understand the meaning of communication. The second thing I would suggest that people think about in terms of communicating in organizations is that communication creates relationships. A lot of people think all communication is about getting your point across, making sure that youre clear in your communication. I take a bit of a different view. Communication creates the relationships that you have with people. Theres that old saying, sticks and stones may break my bones but words will never harm me. It is a terrible, terrible axiom because the words we do use can harm people because they create different relationships. So how you talk does matter. The third thing I would simply say is that people need to be curious about communication. I think one of the biggest problems people run into in organizations when they communicate is they think they know what the other person means. Many times we act on false assumptions. You need to get curious about what people are saying and ask questions like, well, what do you mean by that? and where do you think this is going to take us? and just a number of different questions to sort out what it is that people are meaning because we come from different kinds of experiences, different backgrounds and we need to really understand what a person means by the words that they use.  [2]   4. DIFFERENCES BETWEEN ORAL AND WRITTEN COMMUNICATION Communication requires the transfer of informations from one person to another, and this kind of transfer of informations is dynamic. Writing is a static process of transfer of informations. The written language can be more precise. The choice of written words can be done with greater deliberation and thought. Writer can rewrite his text .The reader can read the text as he wishes. He can do it quickly, slowly or the reader can stop thinking in order to think about the informations that he has just read. The reader also has a possibility of re-reading the text. Speeches should be precise. There needs to be a lot of preparations and compresion for oral communication to be precise. When a word is once spoken it cant be retracted. The speaker can only apoligize for a mistake. One can read from a written text and can achieve with it the same degree of verbal precision as written communication. In most cases audience finds speech-reading uninteresting and boring. Oral communication is considered to be effective in expressing meaning to an audience. During the speech a few signals are available to a speaker: gestures, intonation,ninflection, volume, pitch, pauses, movement, visual cues as appearence The speaker has more control over the informations that the listener will hear than the writer has over what the reader will read. Reading an audience is a systematic and cumulative endeavor unavailable to the writer. As one speaks, the audience provides its own visual cues about whether it is finding the argument coherent, comprehensible, or interesting. Speakers should avoid focusing on single individuals within an audience. 5. COMMUNICATION COMPETENCE The notion of communicative competence was introduced by dell Hymes in the late 1960s. Children grow up in the midst of verbal interactions in which they need to pay attention not only to grammatical form (the order of words and their inflection) but also to the implications, for themselves and other participants, of what is said. To the extent to which linguistic research is concerned with the association between forms and meanings, it should recognize that any linguistic expression is associated with and often reproduces a set of attitudes, values, and beliefs about the world. To be a fluent speaker means to be able to recognize and exploit such attitudes, values and beliefs. Furthermore, language is often only one of the codes used for communication. Speakers complement, refine, and sometimes neutralize or even contradict the meaning of words they use by relying on gestures and eye gaze, and by the use of tools and other material resources around them. The notion of communicative competence was also meant to encourage the study of different codes and modes of communicating within and across situations. Although the term competence echoes Chomskys use of the same word, the addition of the adjective communicative broadens the object of inquiry. Furthermore, while Chomskys notion of competence refers to an abstract set of (typically unconscious) rules-based on an innate language faculty and independent of actual usage-Hymes notion of competence refers to the knowledge necessary for adequately communicating in real life situations. Hence the attention to what speakers actually do with language that is implicit in the notion of communicative competence is not only a methodological but also a theoretical concern. That is, it not only allows us to start from what people do with language in order to infer what they need to know, but alsorecognizes that knowledge of language is gained from participation in actual communicative events, implying the simultaneous relian ce on a number of semiotic resources and the collaboration of other participants. In this sense, Hymes notion of competence is not separate from the notion of performance the use of language as it is in Chompskys model. The introduction of the notion of communicative competence was consistent with Hymes earlier call for a new interdisciplinary field, the etnography of communication (sometimes called the etnography of speaking). This field is dedicated to the empirical study of patterns of speaking in different communities around the world. Theoretically, it draw from a number of traditions, most notably Roman JakobsonS functionalism as represented in his speech event model, from which Hymes derived his notion of communicative event, which is a unit of analysis conceived for the study of communicative competence. In This framework, speaker competence is no longer defined exclusively in terms of their knowledge of grammatical rules, but is also defined in terms of the range of communicative events in which they can participate. Such a range defines persons repertoire within a given community. 6. MOST USUAL MISTAKES IN ORAL COMMUNICATION Commuincators in the process of communication are influenced by several inputs and a deficiency in the quality of any of these inputs can prevent successful communication. According to Adler and Rodman(2006), communicators personal and social background, perception of self and others and verbal and non-verbal language skills are the main inputs influencing the process of communication. Communicators personal background factors such as age, motivation, literacy, and social class make the language learning experience of all individuals unique.  [3]   There are a few factors which can affect oral communication: Language difficulties The use of jargon Accent Physical condition affecting speach or hearing Environmental condition Grammar problems If the persons dont speak the same language fluently, normal conversation is hard to expect. Evidently they will have troubles in understanding each other. Here are a few english words whic are most commonly misspronounced: Archipelago Arctic Accessory Athlete Candidate Chaos Clothes Dilate Height Heinous Hierarchy Illinois Interpret Miniature mischievous Orient Old-fashioned Picture precipitation Drowned Preventive Pronunciation Prostate Jewelry Library Medievel Accent is also one of the factors that can strongly affect the communication between people. If two persons have different accents their communication may not be succesful. Their differences in speach may create misunderstandings. Physical condition affecting speach or hearing is one of the important factors that can influence normal communicaton. If persons who are communicating have a speech or hearing impairment the way that they communicate may have to be adapted. One of the factors by which oral communication is affected is enviromental condition. If persons are communicating in noisy conditions there might be the possibility that one of them miss the important information. In this conditions normal communication is very hard to achieve. Jargon is one of the factors that can affect communication. Especially in working situations people use words and phrases that mean something to them and their colleagues in their workplace or industry but which could be a foreign language to others. Grammar problem is important factor which affects communication. If people lack knowledge of grammar it is hard for them to create sentence, and it is hard to have normal communication in this conditions. There are few most common mistakes that people usually make. Here are the examples of a few gramaticaly incorrect sentences: The man which works here is from Brazil. Ana is married with a doctor. John must to call Isabel immadiately. Although it was snowing,but we had a good time. I like very much chocolate. He can to walk. Where I can find this person? (Where can I find this person?) I have been here since five months. The room is not enough big. (The room is not big enough.) There is nine boys in the bus. We didnt see nobody. He leaves in 6:00 am. Do you like a cup of coffee? Nick should not to drink alcohol 7. IMPROVEMENT OF ORALCOMMUNICATION No matter how good one knows the language, during the oral communication people make mistakes. These mistakes happen from different reasons. Even though oral communication is an effective way of receiving your message across to your listeners, it can also create misunderstanding, misinterpretation and confusion. This happens because each person has his own way of interpreting even when you speak the same language as your listener. There are a few ways to avoid this problems and to improve oral communication. One of the ways to improve oral communication is reading (books and other scriptures). If the person wants to avoid the problem of misunderstanding then it would be good to use simple words that can be clearly understood. Also in order to improve oral skills it is recomandable for a person to be a good listener, because effective communication includes speaking and active listening. If a person listens carefully , what the speaker is talking about, he improves his understanding. If a person wants his listeneres to understand the message it is good to tell a story, joke or read a quote. Jokes usually help people to relax, and in that way their mind will be more open to listen to a speaker. The way in which the story is told can affect the thinking of the listeners. Asking the right questions is also helpful in order to improve communication. A à ¢Ã¢â€š ¬Ã… ¾Why questions gives a person a lot of reasons, excuses, understanding and explanations. à ¢Ã¢â€š ¬Ã… ¾How questions set someones brain thinking for a solution, useful information and a strategy. By asking questions one can create a positive debate and interaction that will benefit all involved. If a speaker wants his speech to be good he should think and prepare before he speaks.. Misunderstanding can occur if a speaker exppresses himself without thinking and allows his emotions to take over. It is good for a speaker to create thoughts in his mind first and then to tell them aloud. Communication can be considered effective if a speaker reduces the use of verbal pauses. If a speaker often stops his sentence and adds à ¢Ã¢â€š ¬Ã… ¾ah, à ¢Ã¢â€š ¬Ã… ¾um, à ¢Ã¢â€š ¬Ã… ¾well it can be very irritating to his listeners. To solve this problemm its good for a speaker to record his own voice and in that way he will be aware of all the mistakes he does during the speech. In this we way the speaker can improve his style of speaking. To have an effective commnication speaker should use words and phrases with care. Speaker shouldnt allow himself that people assume what he is trying to say. Speaker should speak with specifity, and words like never, ever, all, or always need to be avoided. One of the characteristics that speaker should posses is honesty, because if the speaker is promising something to people that is not possible it will break down any trust that the speaker has developed. It is also recomandable for a speaker to relax while speaking. It is important that one doesnt speak in hurry, because that could create impresion that the speaker is confused. Eye contact is also important. If the speaker is facing a crowd he shouldnt look at one person for more than 5 seconds. 8.CONCLUSION From this paper we could learn the most common mistakes people do during oral communication. Most people think that communication is about transferring informations clearly. Regardless of the context, of who youre talking to and of the situation, you need to be clear in your communication. I think thats a misperception because there are times at which being ambiguous can be very effective in your communication. I think one of the reasons that this is a misperception-that we need to be clear-is that our dominant way of thinking about communication is that its about getting your point across, or what some people call transferring information. I think we need to think about communication differently and change the perception of what communication is, that communication is about creating relationships, whether thats creating romantic relationships, whether thats creating relationships in a team, in the workplace, say between an employee and his or her boss, or whether thats creating relationships among nations or countries. It is good to think about communication as a process of creating relationship. So how we communicate creates different things. I think one of the biggest misperceptions is thinking that clear communication is good communication. And I think the reason we think that is because we have a model of communication that says that communication is about transferring information. I think that misperception goes away if we change our perception to one in which communication creates relationships.

George’s Life Sacrifice in Gloria Naylor’s Mama Day Essay -- Gloria Na

George’s Life Sacrifice in Gloria Naylor’s Mama Day George and Ophelia, two characters in Gloria Naylor’s Mama Day, have a complex yet intimate relationship. They meet in New York where they both live. Throughout their hardships, Ophelia and George stay together and eventually get married. Ophelia often picks fights with George to test his love for her, and time after time, he proves to her that he does love her. Gloria Naylor uses George as a Christ figure in his relationship with Ophelia to eventually save her life. While visiting her relatives in Willow Springs, Ophelia becomes deathly ill as a result of evil forces on the island. George tries to think of ways to save her, but he cannot get to a real doctor. The real doctors are across the bridge, which has been destroyed by the hurricane. George helps with rebuilding the bridge practically 24 hours a day, but eventually he becomes disgusted with the others who are working on the bridge, believing that they work too slowly. In his frustration, he speaks in his mind retrospectively to Ophelia: â€Å"If there was a boat ...

As the best form of American Democracy Essay

The world of media is known to be the most important branch of the society which has the ability to influence different types of people due to the immediate access of technologies present in the current world. Individuals have different view points and priorities in which they believe that must be well represented to show the people that such topic must be addressed and given the proper acknowledgement. More and more people are able to show their view points through the use of the television, internet and the likes. There are individuals who only blog and write different articles but their impact has immense to the view points of individuals who are concern are also the same. In the past the media had created its own type of criteria of what not and what should not be. As we, the views see the former and veteran news anchors we often reflect that they are mostly stiff and very limited in the manner of their reporting. In the news broadcasting we often see veteran news anchors that have gained a lot of awards and confidence due to the credibility and long lived career they have. Thus, most of these anchors have entailed the importance of media from the old perspective which had been acknowledged many decades ago. However, due to the technological advancements of internet, computers, mobile phones and the likes, there had been a new trend in adding up information for people the conservatism and rules which must be adhered are now gone. Thus, the media had created a change been the Old Media and the New Media. Old Media and New Media The Old Media is known as the types of media which had been utilized by different personalities such as newspapers, television, radios, movies, books and the likes. These mediums are utilized in order to disseminate to people the current events, criticisms, issues which are in the trend during a certain amount of time. Therefore, the old media are types of materials which are utilized without the use of the latest technologies. The old technologies which use papers, radios and television are the old and classic style of presenting audiences what they want and need to hear from the government and other events which is up to date and certified as accurate. (Agincourt Computing, 2005) The New Media one the other hand is the latest innovation which is utilized by the any individual who is in the entertainment or media industry. The type of media which is mostly utilized by the new media is the internet which is sometimes described as unreliable, and could be effortlessly manipulated by anyone. However, due to the availability of the new media, sometimes the old media is already neglected by the people most especially the youth. The youth are currently much exposed to the effortless access of information in the internet rather than browsing swiftly to every page of the book and absorbing all possible concepts which the author would want to discuss. Most students would want to see a summarized article or a book which they could easily understand without even much analysis from their part. Some people that are on the go prefer some medium that they could put all their information in one single gadget and they could view it whenever they want. Some of these are the main reason for the great interest of people with the new media. However, we must take note that the new media and the old media have its own strengths and weaknesses due to the different aspect that each type of media has. (Vaina, 2008) Almost every individual in the world has their own website and blogsites which they proclaim various types of qualms and experiences during the day or an event. Every one also have Facebook, Twitter, Myspace and the likes that encourages online activities such as gaming, blogging, chatting and etc. as the main activities that individuals with accounts there could use for entertainment. Therefore people all around the world is able to express their emotions through their creativity in arts, and writing, and music while other people has the ability to see such works without even paying a single cent thus, making the internet world a world of different possibilities for those that are actively using it through different means. Though the secrecy and privacy provided by the internet many are capable of doing anything and everything they could possibly want without any types of limitation except for some few which is regulated by some countries. Matt Drugger, New Media and Democracy: In the world of new media, there is one of the many individuals who had became popular is Matt Drudger. He is a media personality who is very controversial in terms of the issues he is raising in terms of the democratic political party in his website. As on article stated he â€Å"is a self-styled seeker of truth, specifically of hidden and obscure truths. If there’s a Drudge brand he’s like to convey, it’s of the relentless, rumpled, ever-vigilant newsman—always connected and plugged into his network of operative (Pachter, 2003). During the controversy of the Bill Clinton and Monica Lewinsky was the epitome of the popularity of his website. As the more controversial he has gotten the type of issues he had been racing are being deeper and deeper compared before. Honestly, we know that nasty kinds of news makes some people interested and sometimes crave for more of it. Many individuals would want something that would ruin the clean slate individuals who are known as the goody-two-shoes and an issue would immediately turn them into vulnerable beasts. This is the action being done by Matt Drudge. Although his work is not as easy as they think it is, Drudge takes his work seriously that he has all the channels in the world that would be able to make him see issues in different parts of the country and the world. Thus, such old news sources will be his secondary sources for his new media business (Pachter, 2003). The internet personality has his website called, www. drudgereport. com which is very much notorious for all the news it publishes online. If the content of the website will be seen links of various types of news website if provided together with the list of the most controversial individuals we could possibly think in politics most especially in the world of Hollywood. In addition to this, he also has the news for the latest technologies, medical improvements, international news and the likes. Any individual who would like to have the latest news could be putting this website in his or her favorite or bookmarked page due to the up to the minute live updates the internet is providing. Although the updates that Drudge is providing are very beneficial for everyone however, the issues that he is creating sometimes disrupt the peacefulness of the American society. It is already a given that many issues will arise within the different aspects of life such as political, environmental, medical and etc. However, there must be a concept of media responsibility in the side of www. drudgesreports. com. Although the new media is not restricted of conservative with regards to information dissemination, it is important that every type of media will become responsible of the power that is given to them. Due to the fact that internet is a location where in anything is possible moral responsibility must be taken into consideration as a part of the rights provided to citizens in the United States. These types of websites are truly the expression of the democratic right of any citizen of the United States. Americans have the power to question and replicate their president in anyway they can. One good example is the Saturday Night Live (SNL) show which incorporates political personalities in their comedic scenes. Most of the time, political leaders are not treated as kindly as they are some political figures who had been part of the many skits the show had done are former President George Bush, President Barack Obama, and many others more. Most of the time, the most controversial individuals are those that are impersonated by great actors. Thus, such show expresses a strong democratic power which is actively exercised by many Americans. If television shows could easy perform such skits inform of millions of people how could a single webpage be stopped by the government. In addition to this, there are too many Americans in the United States and not everyone could be given an immediate response by the government. Through the internet access and web pages such as these the democratic power of Americans is exercised without question. Thus, such type of internet website enhances the ability of Americans to express their views without realizing that they are limited and needing a massive attention from big media corporations such as National Broadcasting Corporation (NBC) and the likes that needed the very unconventional and timely news to be aired in their channels. Through the use such internet websites, any individual could pasture the attention of anyone who is able to connect to the internet and type expound on any topic relevant for their own view point. Thus, the internet have created an a small community where in individuals are not hesitant of proclaiming their views for the reason that they are known to the public and afraid of the scrutiny they will be getting if they would present themselves. In addition, the life threatening possibilities could be done to anyone who contradicts any powerful individual. Although there are no publicized actions which truly contradicts the democracy of media personalities that could be near any human rights violations similar to other countries therefore; the reason for using the new media as a form of expression is also a protection against those who has violent tendencies with regards to this kinds of situation. In conclusion, the old media and the new media are currently the different types of mediums which is utilized and appreciated by all types of people. The old media views the television, books, news papers and the people behind them as the most important entities of media. Moreover the usage of lesser enhanced technology and the classic modes of communication with viewers are categorized as the old media. On the other hand, the new media uses the internet and the continuing technological development build after such as the main medium of its communication with its audience. The internet through blogging, podcasting, Youtube, various internet sites and etc. different information are provided to the various readers that have searched the link of the website. There is easier access, freedom but a great responsibility in publishing anything in the internet. Every sentence that is connected to any type of current issues should be proven and reflected before publishing the article. It is the right of any individual to state anything that is on his or her mind because it is the duty of the people to exercise checking and balancing any activities of any political party or political leader. This responsibility is under the democracy of any citizen of the state. It must be taken into consideration that there are many people in the world who are not able to go in front of a government office to rally and shout out loud against the policies that had been made. Some people chose to write, blog, Myspace, Facebook or Twitter their emotions to make the people understand them or pay attention to their view points. Given that technology is present, why not use it for the greater good? One of those is to exercise democracy and freedom of speech which is provided by the United States of America to all their citizens. Therefore, the website of Matt Drudge is only one of the many internet sites that launch different issues (true or not) that affects different communities most especially the political side of the state. Although some might say that he is very explicit and irresponsible with the news he is presenting but is could not be hindered that this is his right to apply his democratic rights an American Citizen. Taking into consideration the personal lives of the people who are being discussed in this kind of website, individuals who are actively participating must take away the concept of taking matters too personally. Providing too much democracy for people also steps on other people’s rights such as privacy. Therefore, the democracy is rights provided to everyone but a responsibility that must be seriously understand. References Patcher, R. (1 September 2003). â€Å"By linking news sites, Matt Drudge created an Internet success. † Retrieved on 1 April 2009 from http://www. wordsonwords. com/reviews/Drudge903. html. Vaina, D. (15 April 2008) â€Å"New Media Versus Old Media. † Retrieved on 1 April 2009 from http://www. america. gov/st/freepress-english/2008/April/20080513173802WRybakcuH0. 6948358. html Agincourt Computing. (15 December 2004). â€Å"Old Media, New Media. † Retrieved on 1 April 2009 from http://www. aginc. net/media. htm.

The female characters in Great Expectations have the greatest influence on Pip’s development

While it may be true to argue that the female characters of Great Expectations have the most detrimental effect on Pip, it would certainly not be accurate to portray them as having had the greatest influence on his development. Although, the tyrannical Mrs Joe, the embittered Miss Havisham and Pip's ‘beloved' Estella have a large hand in shaping Pip's moral decline, it is without doubt Magwitch and of course, the moral cornerstone of the book, Joe who have the most profound effect on him. As well as this there are the minor male characters of the book such as Wemmick (in his Walworth capacity) and to a lesser extent Jaggers, and their part in restoring Pip to decency. Having said this, it must be acknowledged that in his childhood years it is in fact Mrs Joe who has the most immense influence on Pip's development. Her highly unfavourable â€Å"bring him up by hand,† regime destroys his self-worth, forcing him to develop a real sense of self-resentment. Hear that, be grateful to those who brought you up by hand,† is a deeply ironic statement, for it is Mrs Joe he owes least gratitude to, considering the negative impact she has on Pip's young life. The physical and verbal abuse she expels on him leads to a lack of adequate love and affirmation. â€Å"I'm a common labouring boy, my hands are coarse and my boots are thick†¦ generally I'm in a low lived bad way,† is a bleak outlook that comes from a young boy who has had his self-worth quashed unde r his sister's tyranny. It is of course this lack of self-esteem which is most harmful to Pip's development, forcing him to create the fantasy of reinvention that ultimately leads to his moral decline. However, we cannot forget that regardless of Mrs Joe's predominant influence, Joe, Pip's friend and father figure, is, to an extent, able to have a positive impact on Pip. Primarily, he is able to provide him with what little affirmation possible in such a cold household, establishing a solid friendship with his fellow sufferer, â€Å"We are ever the best of friends ain't us Pip? Furthermore, in terms of moral development, this â€Å"gentle Christian man† is able to instate in Pip the foundations of the values which he must ultimately return to in the peak of his moral decline. Joe the moral mentor, encourages Pip to develop the same goodness he himself possess, by teaching him number of vital lessons such as compassion through the quote â€Å"We don't know what it is you have done, but we wouldn't have you starve to death for it, poor fellow miserable creatur, – would us. ‘ and the importance of honesty through â€Å"(telling lies) ain't the way to get out of being common. Hence Joe is able to have some effect on Pip in his childhood years. Once exposed to the world of Satis house it is the influence of Miss Havisham and Estella that largely occupies his life during the ‘expectation' years. We see the manner in which Miss Havisham plays further on his lack of self-worth, using Estella to belittle him and â€Å"break his heart†; â€Å"He's a coarse and common labouring boy. † This lack of self-worth combines with the glamour of their lifestyle which mesmerizes Pip and leads him to create the fantasy of reinvention. It is the initial meeting at Satis house, therefore, that consequently sees Pip commence his deluded journey in the pursuit of false ideals. Estella also becomes a major contributor to this fantasy, as Pip is naively led on by her and is blinded by her beauty, failing to realize what truly lies beneath. Thus it could be said that Miss Havisham and Estella have a large part in negatively influencing Pip and fuelling his desire to become a ‘gentleman'. In contrast, we see that it is the male character Magwitch who initiates the moral redemption in Pip. Although initially Pip holds him with abhorrent disgust, as time moves on he is forced to under go a major reality check in assessing his idle and wasteful lifestyle. Magwitch is able to extract from Pip the core values he exhibited in his childhood years, in particular the quality of compassion. Towards the end of their relationship we have the declaration from Pip â€Å"May I be as true to you as you have been to me. † indicating the profound effect this common convict has had in returning Pip from the pretentious prig to the morally decent character we are able to respect. Wemmick and to a much lesser extent Jaggers are also able to provide examples of this compassion which help Pip towards his return to decency. During his reality check Pip is also provided with the unselfishness of Wemmick in his Walworth capacity. His caring of the age and the simple idealistic life he leads Pip to open his eyes to true gentility. In a much more ambiguous way we have Jaggers. The revelation of his secretive rescue of Estella from the criminal milieu provides Pip with a compassionate side of Jaggers not revealed before. These examples dramatically influence his development, encouraging his shift back to integrity. However, it is once more Joe who is able to most profoundly influence Pip, completing his moral redemption and providing him with the model of selflessness and compassion. Pip suffers from a mystery illness which is highly symbolic of a phase of personal disassembly, in which he questions himself and rebuilds his character. It is Joe who appears as the saviour, nursing him back to health and in the process teaching Pip that true gentility comes only with qualities such as humility, honesty and most importantly the ability to love and be loved. God bless this gentle Christian man,† is a defining moment in Pip's development as he is finally able to recognise Joe's nobility. Thus without doubt we are left to see that it is the male character Joe who has the most intense influence on Pip's character. It is clear to see therefore that it is instead although the female characters do have quite a detrimental effect on Pip's development, it is instead the male characters and lead by Joe who have the greatest influence. In particular these male characters have quite a substantial hand in helping Pip re-establish his moral decency and hence redeem himself in the eyes of the reader.

I Hear America Essays - Euphoriants, Heroin, Morphinans, Morphine

I Hear America Essays - Euphoriants, Heroin, Morphinans, Morphine I Hear America I hear Americas plead for a place of tranquility, far and bright as the sun. A mom crying for a black son, gone to the cruel actions of racism. A child screaming as a bullet let loose, another innocent classmate lost. A boy watching his friends life slip away to heroin, cocaine, and A daughter hiding in the dark closet, avoiding the hard blows of her alcoholic father. A dad waiting in the ER room, after a disturbing phone call on a drunk driver accident. A girl numbed by fear, too nave to understand the disease a filthy man had given her. I see America dream of an insubstantial place.

Robert Brownings My Last Duchess Essay Example | Topics and Well Written Essays - 500 words

Robert Brownings My Last Duchess - Essay Example In this manner she is finally entirely within his grasp. In life the Duchess had been free-spirited and blessed with a sunny disposition which had irked the Duke as he felt he could not suppress and possess her completely. In the finishing lines of the poem the Duke makes an allusion to Greek mythology which further illustrates his manic need for control and his inherent pride-"Notice Neptune, though/Taming a sea-horse, thought a rarity/Which Claus of Innsbruck cast in bronze for me! The poem is written using iambic pentameter and rhymed couplets. This sets the tone for the poem, and the language thus used gives the reader an impression of power wielded by the narrator. Another poetic device called the enjambment is used so that the lines at times have an unfinished feel creating a hanging effect, and ultimately creating a mood of apprehension as the Duke makes his chilling revelations. He is a compelling narrator and the words he uses have a subtle yet forceful quality to them.

Assignment 1 Example | Topics and Well Written Essays - 500 words - 12

1 - Assignment Example ssue is presently fronting Ashinabe comprise economic development to reduce unemployment, the protection of the wild rice industry from commercial growers, improved medical to battle ailment like diabetes and alcoholism. Also, better management of natural resources, safeguard of treaty of rights and accomplishment of sovereignty as well as amplified prominence on advanced learning to train professional and recommence cultural bonds. Traditionally, health issues affected Ashinabe when they first met the non-Native people suffered from epidemics such as smallpox. Woman married immediately after puberty at age 14 and 15 while boys married as they could able to support family and intermarriages was acceptable as well as polygamy. During birth, parents appointed an elder to give the baby the sacred or dream name and tended to teach by aunts, seniors and mothers at seven years and boys (Hilger 37). Boys were consequently taught hunting and fishing skills by men as girls learned domestic skills from seniors and women with moral values taught through examples and storytelling. Culture of was altered through Anishinabe contact with the non-Native Americans. Trade deepened as Anishinabe hinges on traded goods rather than clothing and utensils. Reservation circumscribed their seasonal movement, formalized education eliminating children from families and the government’s relocation policies disseminated tribe memberships. The culture currently experiencing a rebirth as natives and non-natives are studying Anishibale botany, crafts, myths, and religion. Wild ricing is still valued, even sacred, and constituent of culture in spite that the decline of munificent harvest Anishinabe is fretful about the degradation of the environment by industry and mismanagement. Wild rice harvesting has suffered a setback of alteration in water levels, water pollution, and boat traffic, incursion of alien species of animals and plants as well as housing construction. Logging enterprise have